Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis.
Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis.
复制标题
自体骨髓单核细胞移植可调节兔颈动脉粥样硬化模型的炎症。
DOI：
10.1159/000449201
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发表时间：
2016
影响因子：
1.7
通讯作者：
Wang,Jian
中科院分区：
文献类型：
--
作者：
Cui,Kefei;Wang,Min;Yu,Lie;Ren,Xiao;Cui,Hui;Yu,XiaoFang;Hou,Suyun;Fu,Chao;Wang,Jian
目的：众所周知，炎症在动脉粥样硬化的发生发展中起关键作用，骨髓单个核细胞(BMMNCs)移植可抑制缺血性疾病模型中的炎症反应。在此，我们探讨了自体骨髓单个核细胞移植是否可以通过减轻炎症反应来阻止动脉粥样硬化的进展。方法和结果：采用空气干燥加高脂饮食8周的方法建立兔动脉粥样硬化模型。将1×107个BrdU标记的BMMNCs或等体积的载体经耳缘静脉注入兔体内。使用超声成像方法，我们发现，与赋形剂治疗组相比，自体BMMNC治疗组的动脉粥样硬化斑块面积显著减少(p<；0.05)。苏木精-伊红染色进一步证实了上述结果。逆转录聚合酶链式反应结果显示，与载体组相比，骨髓单个核细胞移植组IL-6和CD147的表达显著降低，而IL-10和转化生长因子-β的表达显著增加(p<；0.05)，这与Western印迹结果一致。结论：自体骨髓单个核细胞移植延缓了动脉粥样硬化的发展，其机制可能与减轻炎症反应有关，这可能是治疗颈动脉粥样硬化的新途径。
Objective:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the progression of atherosclerosis by the alleviation of inflammatory responses in a rabbit model of carotid artery atherosclerosis.Methods and Results:The atherosclerotic rabbit model was established by air desiccation followed by a high-cholesterol diet for 8 weeks. Then, 1 × 107BMMNCs labeled with BrdU or an equal volume of vehicle were injected into the rabbits via the ear vein. Using an ultrasonographic imaging method, we found that autologous BMMNC treatment significantly decreased the area of atherosclerotic plaques compared to the vehicle-treated group (p < 0.05). The results were further confirmed by hematoxylin-eosin staining. RT-PCR results demonstrated that BMMNC treatment significantly reduced the expression of interleukin (IL)-6 and CD147 but increased the expression of IL-10 and transforming growth factor-β compared with vehicle treatment (p<0.05), which was consistent with Western blot results.Conclusions:Transplantation of autologous BMMNCs delays the development of atherosclerosis, most probably via the attenuation of inflammatory responses, which could be a new approach for treating carotid atherosclerosis.