Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea.
Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea.
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DOI：
10.1099/ijs.0.64414-0
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发表时间：
2006-11
影响因子：
2.8
通讯作者：
L. Ten;Qing-mei Liu;W. Im;Myungjin Lee;Deok-Chun Yang;sung-taik lee
中科院分区：
文献类型：
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作者：
L. Ten;Qing-mei Liu;W. Im;Myungjin Lee;Deok-Chun Yang;sung-taik lee
从韩国抱川省人参田的土壤样品中分离出一种革兰氏阴性、严格需氧、杆状、非运动、非孢子形成的细菌菌株，命名为 Gsoil 104T，并使用多相方法进行分类学表征。根据16S rRNA基因序列相似性，菌株Gsoil 104T属于鞘氨醇杆菌科，与Pedobacter africanus DSM 12126T (97.0%)、Pedobacter caeni LMG 22862T (96.9%)、Pedobacter Cryoconitis DSM 14825T (96.8%)和Pedobacter有亲缘关系。 肝素 DSM 2366T (96.6%)。与任何其他具有有效公布名称的Pedobacter物种的系统发育距离大于3.4%（即<96.6% 16S rRNA基因序列相似性）。 DNA-DNA 杂交实验表明，菌株 Gsoil 104T 与其系统发育最接近的菌株之间的 DNA-DNA 相关性值低于 37%。基因组DNA的G+C含量为43.6mol%。主要的呼吸醌是 MK-7。主要脂肪酸为C16:1omega7c、iso-C15:0、C16:0、iso-C17:0 3-OH和iso-C15:0 2-OH。这些化学分类学数据支持菌株 Gsoil 104T 属于土壤杆菌属。根据其表型特性和系统发育的独特性，Gsoil 104T 菌株代表了足细菌属中的一个新物种，其名称为足细菌人参皂甙 sp。十一月被提议。模式菌株是 Gsoil 104T (=KCTC 12576T=LMG 23399T)。
A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 104T, was isolated from a soil sample from a ginseng field in Pocheon Province (South Korea) and was characterized taxonomically by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 104T was shown to belong to the family Sphingobacteriaceae, being related to Pedobacter africanus DSM 12126T (97.0%), Pedobacter caeni LMG 22862T (96.9%), Pedobacter cryoconitis DSM 14825T (96.8%) and Pedobacter heparinus DSM 2366T (96.6%). The phylogenetic distance from any other Pedobacter species with a validly published name was greater than 3.4% (i.e.<96.6% 16S rRNA gene sequence similarity). DNA-DNA hybridization experiments showed that values for DNA-DNA relatedness between strain Gsoil 104T and its phylogenetically closest neighbours were below 37%. The G+C content of the genomic DNA was 43.6 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were C16:1omega7c, iso-C15:0, C16:0, iso-C17:0 3-OH and iso-C15:0 2-OH. These chemotaxonomic data support the affiliation of strain Gsoil 104T to the genus Pedobacter. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 104T represents a novel species in the genus Pedobacter, for which the name Pedobacter ginsengisoli sp. nov. is proposed. The type strain is Gsoil 104T (=KCTC 12576T=LMG 23399T).