Changes in the stiffness of human mesenchymal stem cells with the progress of cell death as measured by atomic force microscopy
Changes in the stiffness of human mesenchymal stem cells with the progress of cell death as measured by atomic force microscopy
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DOI：
10.1016/j.jbiomech.2013.12.004
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发表时间：
2014-02-07
影响因子：
2.4
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Nikolaev, Nikolay I.;Mueller, Torsten;Liu, Yang
本文报告了通过 AFM 测量的人间充质干细胞 (hMSC) 硬度随细胞死亡进展的变化的观察结果。膜受损的 hMSC、死细胞和活细胞在冷藏 24 小时后用膜联蛋白 V 和碘化丙啶标记，然后进行 AFM 测量并得出细胞的杨氏模量。存活的 hMSC 的杨氏模量 (E) 在 0.81-1.13 kPa 范围内，并且当选择不同的测量位置时观察到一致的测量结果。膜部分受损的细胞的 E 为 0.69 +/- 0.17 kPa 或在 2.04-4.74 kPa 范围内，具体取决于测量位置。随着膜完整性的丧失，尽管不同位置之间测量的 E 没有变化，但观察到细胞硬度的混合情况，如 E 低至 0.09 +/- 0.03 kPa、中间范围为 4.62 +/- 0.67 kPa、最高可达 48.98 +/- 19.80 kPa 的细胞所示。随着细胞死亡的进行，观察到呈现更多颗粒状外观的细胞的硬度最高；对于具有液泡外观的细胞来说，硬度也是最低的。这项研究的结果表明，细胞硬度随着细胞死亡的进展而显着改变。 (C) 2013 作者。由爱思唯尔有限公司出版。保留所有权利。
This note reports observations of the change of stiffness of human mesenchymal stem cells (hMSCs) with the progress of cell death as measured by AFM. hMSC with impaired membrane, dead and viable cells were labelled with Annexin V and Propidium Iodide after 24 h cold storage, followed by AFM measurement and Young's modulus of cells was derived. Viable hMSCs have a Young's modulus (E) in the range of 0.81-1.13 kPa and consistent measurement was observed when different measurement locations were chosen. E of cells with partially impaired membrane was 0.69 +/- 0.17 kPa or in the range of 2.04-4.74 kPa, depending upon the measurement locations. With the loss of membrane integrity, though there was no variation on measured E between different locations, a mixed picture of cell stiffness was observed as indicated by cells with E as low as 0.09 +/- 0.03 kPa, in a mid-range of 4.62 +/- 0.67 kPa, and the highest of up to 48.98 +/- 19.80 kPa. With the progress of cell death, the highest stiffness was noticed for cells showing a more granular appearance; also the lowest stiffness for cells with vacuole appearance. Findings from this study indicate that cell stiffness is significantly altered with the progress of cell death. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.