Gallus expression in situ hybridization analysis: A chicken embryo gene expression database
Gallus expression in situ hybridization analysis: A chicken embryo gene expression database
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DOI：
10.1093/ps/86.7.1472
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发表时间：
2007-07-01
期刊：
影响因子：
4.4
通讯作者：
Darnell, D. K.
中科院分区：
文献类型：
--
作者：
Antin, P. B.;Kaur, S.;Darnell, D. K.
随着鸡基因组测序基本完成，重要的工作集中在基因注释上，包括获取有关基因表达模式的信息。鸡胚胎非常适合通过体内原位杂交基因表达分析提供详细的时间和空间表达信息。我们开发了 Gallus 表达原位杂交分析 (GEISHA) 数据库和用户界面 (http://geisha.arizona.edu)，作为鸡胚胎原位杂交照片和元数据的集中存储库。本报告描述了 GEISHA 数据库和网站的设计和实施，并说明了其对生物医学和家禽科学界研究人员的有用性。还介绍了最近对鸡胚胎中 microRNA 表达的综合表达分析的结果。
With sequencing of the chicken genome largely completed, significant effort is focusing on gene annotation, including acquiring information about the patterns of gene expression. The chicken embryo is ideally suited to provide detailed temporal and spatial expression information through in situ hybridization gene expression analysis in vivo. We have developed the Gallus expression in situ hybridization analysis (GEISHA) database and user interface (http://geisha.arizona.edu) to serve as a centralized repository of in situ hybridization photos and metadata from chicken embryos. This report describes the design and implementation the GEISHA database and Web site and illustrates its usefulness for researchers in the biomedical and poultry science communities. Results from a recent comprehensive expression analysis of microRNA expression in chicken embryos are also presented.