Comparative analysis of plant immune receptor architectures uncovers host proteins likely targeted by pathogens.
Comparative analysis of plant immune receptor architectures uncovers host proteins likely targeted by pathogens.
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DOI：
10.1186/s12915-016-0228-7
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发表时间：
2016-02-19
期刊：
影响因子：
5.4
通讯作者：
Krasileva KV
中科院分区：
文献类型：
--
作者：
Sarris PF;Cevik V;Dagdas G;Jones JD;Krasileva KV
植物部署免疫受体来检测病原体衍生的分子并启动防御反应。细胞内的植物免疫受体称为核苷酸结合的富亮氨酸重复序列(NLR)蛋白，它含有一个中心核苷酸结合(NB)结构域，随后是一系列富含亮氨酸的重复序列(LRR)，是植物防御反应的关键启动者。然而，最近的研究表明，具有非规范结构域结构的NLRs在植物免疫中发挥着重要作用。这些复合免疫受体被认为是由NLR和额外的结构域之间的融合而产生的，这些结构域充当病原体衍生的效应蛋白的“诱饵”，从而使病原体能够识别。有几个名称被用来描述这些蛋白质，包括“集成诱饵”和“集成传感器”。我们采用并主张“集成域”或NLR-ID，它描述了融合的产物，而没有指定一个通用的行动模式。我们已经扫描了现有的植物基因组序列的全谱NLR-ID，以评估潜在的传感器/诱骗结构域在开花植物中整合的多样性，包括19个作物物种。我们手动管理小麦和甘蓝，并在野生和栽培小麦品种中实验验证了NLR-ID的一个子集。我们研究了发生在多个植物家族中的NLR融合，并发现一些结构域显示出跨谱系的重复整合。与NLR融合的结构域与先前确定的病原体靶标重叠，证实它们是病原体的诱饵。虽然一些整合的结构域以前被认为与抗病有关，但另一些结构域为植物病原体的持久抗性工程提供了新的靶点。我们已经建立了一个健壮的、可重复的管道，用于检测跨物种的植物免疫受体中的可变域结构。我们假设，我们揭示的NLR-ID为病原体靶向的宿主蛋白提供了线索，并且这些信息可以用于发现新的抗病来源。本文的在线版本(doi：10.1186/s12915-0160228-7)包含补充材料，授权用户可以使用。
Plants deploy immune receptors to detect pathogen-derived molecules and initiate defense responses. Intracellular plant immune receptors called nucleotide-binding leucine-rich repeat (NLR) proteins contain a central nucleotide-binding (NB) domain followed by a series of leucine-rich repeats (LRRs), and are key initiators of plant defense responses. However, recent studies demonstrated that NLRs with non-canonical domain architectures play an important role in plant immunity. These composite immune receptors are thought to arise from fusions between NLRs and additional domains that serve as “baits” for the pathogen-derived effector proteins, thus enabling pathogen recognition. Several names have been proposed to describe these proteins, including “integrated decoys” and “integrated sensors”. We adopt and argue for “integrated domains” or NLR-IDs, which describes the product of the fusion without assigning a universal mode of action. We have scanned available plant genome sequences for the full spectrum of NLR-IDs to evaluate the diversity of integrations of potential sensor/decoy domains across flowering plants, including 19 crop species. We manually curated wheat and brassicas and experimentally validated a subset of NLR-IDs in wild and cultivated wheat varieties. We have examined NLR fusions that occur in multiple plant families and identified that some domains show re-occurring integration across lineages. Domains fused to NLRs overlap with previously identified pathogen targets confirming that they act as baits for the pathogen. While some of the integrated domains have been previously implicated in disease resistance, others provide new targets for engineering durable resistance to plant pathogens. We have built a robust reproducible pipeline for detecting variable domain architectures in plant immune receptors across species. We hypothesize that NLR-IDs that we revealed provide clues to the host proteins targeted by pathogens, and that this information can be deployed to discover new sources of disease resistance. The online version of this article (doi:10.1186/s12915-016-0228-7) contains supplementary material, which is available to authorized users.