The repair of brain lesion by implantation of hyaluronic acid hydrogels modified with laminin
The repair of brain lesion by implantation of hyaluronic acid hydrogels modified with laminin
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DOI：
10.1016/j.jneumeth.2005.04.016
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发表时间：
2005-10-15
影响因子：
3
通讯作者：
Lee, IS
中科院分区：
文献类型：
--
作者：
Hou, SP;Xu, QY;Lee, IS
将层粘连蛋白修饰的透明质酸（HA）水凝胶植入大鼠脑内，观察其对中枢神经系统损伤的修复作用。合成了交联HA水凝胶，并对其性能进行了分析。层粘连蛋白是一种细胞外基质蛋白，参与神经元的发育和存活，固定在水凝胶的骨架上。将未修饰的和用层粘连蛋白修饰的水凝胶植入大鼠中机械产生的皮质缺损中，然后评价其改善组织重建的能力。植入6周和12周后，脑切片用Nissl和Glees染色以显示神经细胞体和纤维，用DAB组织化学检测血管，以及用免疫细胞化学识别GFAP。并作电镜和扫描电镜观察。结果表明，所合成的HA水凝胶具有与脑组织相似的力学性能和流变行为。将多孔水凝胶植入病变皮层后，形成了一种支架，可以支持细胞浸润和血管生成，同时抑制胶质瘢痕的形成。此外，层粘连蛋白修饰的HA水凝胶可以促进神经突起的延伸。本研究结果提示，组织工程技术可能为中枢神经系统损伤的修复铺平道路。(c)2005 Elsevier B.V.保留所有权利。
The hyaluronic acid (HA) hydrogels modified with laminin were used for implantation in rat brain in present study, in order to investigate its effects in reparation of injury in the CNS. Cross-linked HA hydrogels were synthesized and their characteristics were analyzed. Laminin, an extracellular matrix protein, which participates in neuronal development and survival, was immobilized on the backbone of the hydrogels. Hydrogels unmodified and modified with laminin were implanted into cortical defects mechanically created in rats and their ability to improve tissue reconstruction was then evaluated. After 6 and 12 weeks of implantation, sections of brains were processed with Nissl and Glees staining for revealing neural cell bodies and fibers, with DAB histochemistry for detecting the blood vessels, as well as with immunocytochemistry for recognizing GFAP. The sections were also taken to SEM and TEM for ultrastructral examination. The results showed that the HA hydrogels synthesized had mechanical properties and theological behavior similar to the brain tissue. After being implanted into the lesion of the cortex, the porous hydrogels created a scaffold, which could support cell infiltration and angiogenesis, and simultaneously inhibit the formation of glial scar. In addition, HA hydrogels modified with laminin could promote neurite extension. It seems possible that the tissue engineering technique may pave the way to repair injury in the CNS as suggested by the results in present study. (c) 2005 Elsevier B.V. All rights reserved.