Biomolecular Switch Circuits for Molecular Robots using a DNA Strand Displacement Mechanism
Biomolecular Switch Circuits for Molecular Robots using a DNA Strand Displacement Mechanism
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DOI：
10.23919/sice.2019.8859901
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发表时间：
2019-09
期刊：
影响因子：
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通讯作者：
Keisuke Watanabe;T. Nakakuki
中科院分区：
文献类型：
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作者：
Keisuke Watanabe;T. Nakakuki
纳米生物工程的进展最近集中在通过组合生物分子装置建立的分子机器人上。在这项研究中，我们的目标是创建一个DNA开关电路来改变分子机器人的状态。主要目标是在固定时间内激活致动器。本研究中详细介绍的设计的优点是在温和的环境条件下唯一使用链置换反应，有助于分子机器人的实际使用。尽管由于开关电路的性质经常发生不期望的反应，但使用错配的碱基对来试图避免这种情况。仿真和实验都证明了开关电路的正确性。
Advances in nanoscale bioengineering have recently been focusing on molecular robots established by combining biomolecular devices. In this study, we aimed to create a DNA switch circuit to alter the states of a molecular robot. The primary goal was to activate the actuator for a fixed period. The merit of the design detailed in this study was the sole use of strand displacement reactions under mild environmental conditions, contributing to practical use within the molecular robot. Although undesirable reactions often occur due to the nature of the switch circuit, mismatched base pairs were used to try to avoid this. The correct behavior of the switch circuit has been proven by both the simulation and experiment.