Disturbed ratio of erythrocyte and plasma S-adenosylmethionine/S-adenosylhomocysteine in peripheral arterial occlusive disease
Disturbed ratio of erythrocyte and plasma S-adenosylmethionine/S-adenosylhomocysteine in peripheral arterial occlusive disease
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DOI：
10.1016/s0021-9150(00)00449-4
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发表时间：
2001-01-01
期刊：
影响因子：
5.3
通讯作者：
Haefeli, WE
中科院分区：
文献类型：
--
作者：
Loehrer, FMT;Tschöpl, M;Haefeli, WE
与外周动脉闭塞性疾病（PAOD）相关的同型半胱氨酸代谢改变可能通过s -腺苷型半胱氨酸（AdoHcy）的积累以及s -腺苷型蛋氨酸(AdoMet)/AdoHcy比值的改变导致重要甲基化反应的损害。我们测定了61例PAOD患者（年龄49-93岁）和50例健康对照（年龄41-87岁）血浆中AdoMet、AdoHcy及其比值、同型半胱氨酸以及红细胞中AdoMet、AdoHcy及其比值。与对照组相比，患者血浆同型半胱氨酸、AdoMet、AdoHcy几何平均值显著升高（15.5 vs 10.4 μ mol/l**; 107 vs 52.3* nmol/l; 55.0 vs 23.1** nmol/l; *P < 0.01, **P < 0.001），而AdoMet/AdoHcy比值降低（1.92 vs 2.52*）。在红细胞中，与对照组相比，患者adhcy水平升高（309 vs. 205 nmol/l**），而AdoMet （3351 vs. 3732 nmol/l**）和AdoMet/ adhcy比值（11.8 vs. 19.1**）降低。校正肾功能后AdoMet/ adhcy比降低的pad的优势比（OR）在红细胞水平小于或等于14.2时具有显著性(OR为7.1 （6.9-7.2,95% CI）。此外，与对照组相比，患者红细胞压积水平显著降低（0.35 vs. 0.42 l/l**），且与患者红细胞AdoMet/ adhcy比值显著相关。由于AdoMet/AdoHcy的比例与许多酶甲基化反应的活性密切相关，这些结果表明甲基化可能在这些患者中受损。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
Altered homocysteine metabolism associated with peripheral arterial occlusive disease (PAOD) may lead to impairment of vital methylation reactions through accumulation of S-adenosylhomocysteine (AdoHcy) as well as through alteration of the ratio S-adenosylmethionine (AdoMet)/AdoHcy. We determined AdoMet, AdoHcy, their ratio, and homocysteine in plasma as well as AdoMet, AdoHcy, and their ratio in erythrocytes of 61 patients with PAOD (age 49-93) and 50 healthy controls (age 41-87). Geometric mean values of plasma homocysteine, AdoMet, and AdoHcy were significantly increased in patients compared with controls (15.5 vs. 10.4 mu mol/l**; 107 vs. 52.3* nmol/l; 55.0 vs. 23.1** nmol/l, respectively; *P < 0.01, **P < 0.001), while the ratio of AdoMet/AdoHcy was decreased in patients (1.92 vs. 2.52*). In erythrocytes patients exhibited increased levels of AdoHcy compared with controls (309 vs. 205 nmol/l**) whereas AdoMet (3351 vs. 3732 nmol/l*) and the ratio of AdoMet/AdoHcy (11.8 vs. 19.1**) were decreased. The odds ratio (OR) for developing PAOD with decreased AdoMet/AdoHcy ratio after adjustment for kidney function was significant for erythrocyte levels less than or equal to 14.2 (OR, 7.1 (6.9-7.2, 95% CI). Tn addition, hematocrit levels were found to be significantly decreased in patients versus controls (0.35 vs. 0.42 l/l**) and were significantly correlated with the ratio of AdoMet/AdoHcy in erythrocytes of the patients. Since the ratio of AdoMet/AdoHcy is closely linked with the activity of numerous enzymatic methylation reactions, these results suggest that methylation may be impaired in these patients. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.