Dispensability of nanos mRNA localization for abdominal patterning but not for germ cell development
Dispensability of nanos mRNA localization for abdominal patterning but not for germ cell development
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DOI：
10.1016/j.mod.2007.10.004
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Wolff, Lisa J.
中科院分区：
文献类型：
--
作者：
Gavis, Elizabeth R.;Chatterjee, Seema;Wolff, Lisa J.
功能生殖系的发育对物种繁殖至关重要。纳米（nos）基因在种系发育中起着进化保守的作用，也是果蝇腹部图案的必要条件。nos mRNA的一小部分定位于果蝇胚胎后极的种质，在那里它被纳入生殖细胞。胚质相关的nos mRNA被翻译成腹部的nos蛋白梯度，而剩余的未定位RNA被翻译抑制以允许前发育。利用转基因损害nos mRNA的定位和翻译调控，我们表明，只要nos翻译得到适当调节，野生型体模式可以在没有nos mRNA定位的情况下发生。相比之下，nos定位于生殖质，而不是翻译调控，对于nos在发育中的生殖细胞中的功能是必不可少的。我们认为，在产生功能性种系的过程中，nos定位的必要性保留了一个低效的定位机制。2007爱思唯尔爱尔兰有限公司版权所有。
The development of a functional germline is essential for species propagation. The nanos (nos) gene plays an evolutionarily conserved role in germline development and is also essential for abdominal patterning in Drosophila. A small fraction of nos mRNA is localized to the germ plasm at the posterior pole of the Drosophila embryo, where it becomes incorporated into the germ cells. Germ plasm associated nos mRNA is translated to produce a gradient of Nos protein that patterns the abdomen, whereas the remaining unlocalized RNA is translationally repressed to allow anterior development. Using transgenes that compromise nos mRNA localization and translational regulation, we show that wild-type body patterning can ensue without nos mRNA localization provided that nos translation is properly modulated. In contrast, localization of nos to the germ plasm, but not translational regulation, is essential for nos function in the developing germ cells. We propose that an imperative for nos localization in producing a functional germline has preserved an inefficient localization mechanism. (c) 2007 Elsevier Ireland Ltd. All rights reserved.