Regional mapping of human genes for hexosaminidase B and diphtheria toxin sensitivity on chromosome 5 using mouse × human hybrid cells
Regional mapping of human genes for hexosaminidase B and diphtheria toxin sensitivity on chromosome 5 using mouse × human hybrid cells
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使用小鼠 × 人类杂交细胞对 5 号染色体上的己糖胺酶 B 和白喉毒素敏感性人类基因进行区域定位
DOI：
10.1007/bf01539070
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
U. Francke
中科院分区：
文献类型：
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作者：
D. George;U. Francke
小鼠3T3 （TK−）细胞与含有平衡易位的人白细胞融合[ins(3;5) (q27;q13q15)]，其中5号染色体长臂的一部分插入到3号染色体长臂中。两个独立的初级杂交克隆（XVI-10C; XVI-18A）保留了缺失的5号染色体[del(5) (q13q15)]易位产物，并为5号染色体上参与己糖胺酶B （HEXB）和白喉毒素敏感性（DTS）表达的基因的区域定位提供了信息。XVI-10C和XVI-18A克隆均对白喉毒素敏感。对这些克隆的抗毒素衍生物（XVI-10C DTR和XVI-18A DTR）以及未暴露于白喉毒素的XVI-10C和XVI-18A细胞的染色体含量和Hex B活性表达进行了分析。本研究结果为dts定位于5号染色体长臂5q15→5qter区，hexb定位于5pter→5q13区提供了证据。
Mouse 3T3 (TK−) cells were fused to human leukocytes containing a balanced translocation [ins(3;5) (q27;q13q15)] in which part of the long arm of a chromosome 5 has been inserted into the long arm of a chromosome 3. Two independent, primary hybrid clones (XVI-10C; XVI-18A) retained the deleted chromosome 5 [del(5) (q13q15)] translocation product and were informative for regional mapping on chromosome 5 of genes involved in expression of hexosaminidase B (HEXB) and diphtheria toxin sensitivity (DTS). Both XVI-10C and XVI-18A clones were sensitive to diphtheria toxin. Toxin-resistant derivatives of these clones (XVI-10C DTR; XVI-18A DTR) were analyzed for chromosome content and expression of Hex B activity, as were XVI-10C and XVI-18A cells which had not been exposed to diphtheria toxin. The results of this study provide evidence for localization ofDTS to region 5q15→5qter on the long arm of chromosome 5, and localization ofHEXB to region 5pter→5q13.