The chimpanzee model of hepatitis C virus infections.
The chimpanzee model of hepatitis C virus infections.
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DOI：
10.1093/ilar.42.2.117
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发表时间：
2001
期刊：
影响因子：
2.5
通讯作者：
R. Lanford;Catherine B. Bigger;Suzanne E. Bassett;G. Klimpel
中科院分区：
文献类型：
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作者：
R. Lanford;Catherine B. Bigger;Suzanne E. Bassett;G. Klimpel
黑猩猩（Pan troglodytes）是唯一易感染丙型肝炎病毒（HCV）的实验动物。 HCV 感染的黑猩猩模型在非甲型、非乙型肝炎的初步研究中发挥了重要作用，包括对感染临床过程的观察、病毒物理特性的测定以及最终 HCV 核酸的克隆。本综述重点关注黑猩猩在 HCV 研究中的应用的最新情况。黑猩猩模型对于分析 HCV 感染的早期事件至关重要，因为它代表了一个群体，从暴露之时起就可以获得样本，并且对所有暴露的动物进行了检查。因此，黑猩猩代表了一个真正未经选择的种群。相比之下，人类群体通常是根据疾病状态或抗体反应性来选择的，通常包括已经感染数十年的个体。黑猩猩模型对于更好地了解病毒清除所涉及的因素、分析对感染的免疫反应以及疫苗的开发至关重要。 HCV 感染性 cDNA 克隆的开发依赖于黑猩猩的使用，并且在使用反向遗传学来评估病毒复制的关键序列时将继续需要它们。此外，黑猩猩已与DNA微阵列技术结合使用，以探测HCV感染过程中肝脏基因表达的整个变化范围。黑猩猩将继续为丙型肝炎病毒疾病的理解和治疗方式的开发提供重要的方面。
The chimpanzee (Pan troglodytes) is the only experimental animal susceptible to infection with hepatitis C virus (HCV). The chimpanzee model of HCV infection was instrumental in the initial studies on non-A, non-B hepatitis, including observations on the clinical course of infection, determination of the physical properties of the virus, and eventual cloning of the HCV nucleic acid. This review focuses on more recent aspects of the use of the chimpanzee in HCV research. The chimpanzee model has been critical for the analysis of early events in HCV infection because it represents a population for which samples are available from the time of exposure and all exposed animals are examined. For this reason, the chimpanzee represents a truly nonselected population. In contrast, human cohorts are often selected for disease status or antibody reactivity and typically include individuals that have been infected for decades. The chimpanzee model is essential to an improved understanding of the factors involved in viral clearance, analysis of the immune response to infection, and the development of vaccines. The development of infectious cDNA clones of HCV was dependent on the use of chimpanzees, and they will continue to be needed in the use of reverse genetics to evaluate critical sequences for viral replication. In addition, chimpanzees have been used in conjunction with DNA microarray technology to probe the entire spectrum of changes in liver gene expression during the course of HCV infection. The chimpanzee will continue to provide a critical aspect to the understanding of HCV disease and the development of therapeutic modalities.