Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame.
Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame.
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DOI：
10.3389/fpls.2017.00410
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发表时间：
2017
影响因子：
5.6
通讯作者：
Kundu S
中科院分区：
文献类型：
--
作者：
Chowdhury S;Basu A;Kundu S
类渗透调节蛋白（OLP）是PR-5家族的成员，在植物体内介导对生物和非生物胁迫的防御。在芝麻中过表达的OLP基因（SindOLP），增强了对干旱，盐，氧化胁迫和炭腐病原体的耐受性。SindOLP在所有部分中表达并定位于细胞质。转基因植株在长时间干旱和盐胁迫后恢复，与对照植株相比，表现出更少的电解质渗漏，更多的含水量，更长的根和更小的气孔开度。在多重胁迫下，转基因芝麻的渗透调节物质、ROS清除酶、叶绿素含量、脯氨酸和次生代谢产物增加，脂质过氧化作用降低。OLP基因通过增加编码ROS清除酶的三个基因和在JA/ET和SA途径中起作用的五个防御相关标记基因（即Si-Apetala 2、Si-乙烯响应因子、Si-防御素、Si-几丁质酶和Si-奇异果甜蛋白样蛋白）的表达来赋予增加的耐受性。与对照相比，通过整合激活防御信号级联的多个组分，转基因株系在不同胁迫下表现出更大的存活率。这是转基因芝麻的首次报道，也是芝麻中防御相关基因的首次研究。这也是第一次尝试了解的分子机制的基础上的多胁迫耐受性赋予的OLP。
Osmotin-like proteins (OLPs), of PR-5 family, mediate defense against abiotic, and biotic stresses in plants. Overexpression in sesame of an OLP gene (SindOLP), enhanced tolerance against drought, salinity, oxidative stress, and the charcoal rot pathogen. SindOLP was expressed in all parts and localized to the cytosol. The transgenic plants recovered after prolonged drought and salinity stress, showing less electrolyte leakage, more water content, longer roots, and smaller stomatal aperture compared to control plants. There was an increase in osmolytes, ROS-scavenging enzymes, chlorophyll content, proline, secondary metabolites, and reduced lipid peroxidation in the transgenic sesame under multiple stresses. The OLP gene imparted increased tolerance through the increased expression of three genes coding for ROS scavenging enzymes and five defense-related marker genes functioning in the JA/ET and SA pathways, namely Si-Apetala2, Si-Ethylene-responsive factor, Si-Defensin, Si-Chitinase, and Si-Thaumatin-like protein were monitored. The transgenic lines showed greater survival under different stresses compared to control through the integrated activation of multiple components of the defense signaling cascade. This is the first report of transgenic sesame and first of any study done on defense-related genes in sesame. This is also the first attempt at understanding the molecular mechanism underlying multi-stress tolerance imparted by an OLP.