BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins.
BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins.
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BLM（布鲁姆氏综合症蛋白）在细胞周期中其数量、分布以及与其他核蛋白的共定位发生变化。
DOI：
10.1159/000056848
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
German,J
中科院分区：
文献类型：
--
作者：
Sanz,MM;Proytcheva,M;Ellis,NA;Holloman,WK;German,J
BLM是由Bloom综合征(BS)中突变的基因编码的蛋白质，是一种在系统发育上高度保守的DNA解旋酶，在细胞分裂周期中，它在细胞核中的数量和分布发生变化。在许多细胞从有丝分裂中出现时检测不到它，但在G1期变得丰富，并在S、G2和有丝分裂过程中一直如此。BLM广泛分布于整个细胞核，但有时也集中在数量和大小不同的病灶中。它与复制蛋白A(RPA)和早幼粒细胞白血病蛋白(PML)核体短暂共定位，有时进入核仁。这些观察结果支持这样一种假设，即在一些，很可能是几个核酸交易中，BLM在细胞核内的不同分布，在它们发生的时间和地点操纵DNA。具体交易对象(S)尚待确定。虽然功能BLM的核缺失-BS的情况-在人类中显然不是致命的，但其他解旋酶似乎无法完全取代它，见证BS细胞的高度变异性和高度重组。
BLM, the protein encoded by the gene mutated in Bloom’s syndrome (BS), is a phylogenetically highly conserved DNA helicase that varies in amount and distribution in the nucleus during the cell-division cycle. It is undetectable in many cells as they emerge from mitosis but becomes abundant during G 1 and remains so throughout S, G 2, and mitosis. BLM is widely distributed throughout the nucleus but at certain times also becomes concentrated in foci that vary in number and size. It co-localizes transitorily with replication protein A (RPA) and promyelocytic leukemia protein (PML) nuclear bodies, and at times it enters the nucleolus. The observations support the hypothesis that BLM is distributed variously about the nucleus to manipulate DNA in some, very possibly several, nucleic acid transactions, when and where they take place. The specific transaction (s) remain to be identified. Although absence from the nucleus of functional BLM–the situation in BS–obviously is not lethal in the human, other helicases would appear to be unable to substitute for it completely, witness the hypermutability and hyperrecombinability of BS cells.