A cholinergic model synapse to elucidate protein function at presynatic terminals
A cholinergic model synapse to elucidate protein function at presynatic terminals
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DOI：
10.1016/j.neures.2006.12.015
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发表时间：
2007-04-01
影响因子：
2.9
通讯作者：
Mochida, Sumiko
中科院分区：
文献类型：
--
作者：
Ma, Huan;Mochida, Sumiko
大量的蛋白质已被确定在神经末梢和级联的蛋白质-蛋白质相互作用已被认为是参与突触囊泡状态的循环。研究突触前终末蛋白质的功能，目前仅限于鱿鱼巨突触、Held突触萼和海马神经元自突触等少数几种独特的突触。鱿鱼巨突触和Held的萼是有用的，以引入试剂到其大的突触前末梢和海马神经元autapse是一个很好的系统，以修改蛋白质水平的外源DNA或RNA。颈上级神经节（SCG）神经元之间在长期培养中形成的胆碱能突触是研究快速突触的有用模型。大细胞体的轴突与邻近神经元的索马相接触。突触连接的结构使得可以通过在短时间内从细胞体扩散将试剂引入突触前末梢。通过显微注射将外源性cDNA或siRNA引入SCG神经元，使我们能够调节感兴趣的蛋白质的水平或在神经元中表达突变蛋白。在这里，我们描述了使用模型SCG神经元突触来阐明突触前蛋白在介导突触传递中的功能。(c)2007 Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
A large number of proteins have been identified at nerve terminals and a cascade of protein-protein interactions has been suggested to be involved in cycling of synaptic vesicle states. To explore protein function in presynaptic terminals, only a few unique synapses such as the squid giant synapse, the calyx of Held synapse and the hippocampal neuron autapse have been used. The squid giant synapse and the calyx of Held are useful to introduce reagents into their large presynaptic terminals and the hippocampal neuron autapse is a good system to modify a protein level by exogenous DNA or RNA. The cholinergic synapse formed between superior cervical ganglion (SCG) neurons in long-term culture is a useful model for a fast synapse. The axon of the large cell body contacts with soma of neighboring neurons. The architecture of synaptic connections makes it possible to introduce reagents into the presynaptic terminals by diffusion from a cell body within a short time. Introduction of exogenous cDNA or siRNA performed by microinjection into a SCG neuron allows us to modulate the level of the protein of interest or to express mutant proteins in the neuron. Here, we describe use of the model SCG neuronal synapse to elucidate function of presynaptic proteins in mediating synaptic transmission. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.