Chemotransport contributes to the effect of oscillatory fluid flow on human bone marrow stromal cell proliferation
Chemotransport contributes to the effect of oscillatory fluid flow on human bone marrow stromal cell proliferation
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DOI：
10.1002/jor.20637
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Donahue, Henry J.
中科院分区：
文献类型：
--
作者：
Riddle, Ryan C.;Hippe, Karen R.;Donahue, Henry J.
机械负荷产生一组不同的生物物理信号，可以调节骨细胞的活性，但越来越多的证据表明，间质液流量是骨细胞感知的主要信号。因为我们以前证明，振荡流体流增加人骨髓基质细胞增殖，我们调查的贡献流体剪切应力和化学转运，两个刺激诱导的间质液流。在恒定的峰值剪切应力下，流速的变化与振荡流体流诱导的骨髓基质细胞增殖的减少有关，而峰值流体剪切应力的变化没有显著影响。流速对骨髓基质细胞增殖的调节可能归因于ATP释放和细胞内钙信号的变化。我们发现，如果流速降低，同时保持一个恒定的峰值流体剪切应力，骨髓基质细胞释放到细胞外环境的ATP较少。此外，随着流速降低，响应流体流动的细胞减少，细胞内钙浓度增加。这些数据表明，化学转运是骨髓基质细胞响应间质液流动的先决条件。(C)2008骨科研究学会。出版社：Wiley Periodicals，Inc.
Mechanical loads produce a diverse set of biophysical signals that may regulate bone cell activity, but accumulating evidence suggests that interstitial fluid flow is the primary signal that bone cells perceive. Because we previously demonstrated that oscillatory fluid flow increases human bone marrow stromal cell proliferation, we investigated the contribution of fluid shear stress and chemotransport, two stimuli induced by interstitial fluid flow. Alterations in flow rate at a constant peak shear stress were associated with decreases in oscillatory fluid flow-induced marrow stromal cell proliferation, while variations in peak fluid shear stress had no significant effect. Modulation of marrow stromal cell proliferation by flow rate may be attributed to changes in the release of ATP and intracellular calcium signaling. We found that if the flow rate is decreased while maintaining a constant peak fluid shear stress, marrow stromal cells release less ATP into the extracellular environment. Moreover, as the flow rate decreased fewer cells respond to fluid flow with an increase in intracellular calcium concentration. These data suggest that chemotransport is a prerequisite for marrow stromal cells to respond to interstitial fluid flow. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.