Peptide-polymer vesicles prepared by atom transfer radical polymerization
Peptide-polymer vesicles prepared by atom transfer radical polymerization
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DOI：
10.1002/pola.21107
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发表时间：
2005-12-15
影响因子：
--
通讯作者：
van Hest, JCM
中科院分区：
文献类型：
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作者：
Ayres, L;Hans, P;van Hest, JCM
用固相肽化学法制备了Ac-Ser-Ala-Gly-Ala-Gly-Glu-Gly-Ala-Gly-Ser-Gly-OH肽。在从固体支持物中除去肽之前，用α-溴酯部分官能化两个丝氨酸的醇侧基以产生双官能引发剂。该基于肽的引发剂在溶液中用于甲基丙烯酸甲酯的原子转移自由基聚合以产生明确定义的阿坝三嵌段共聚物，其中聚（甲基丙烯酸甲酯）末端嵌段具有1.1kg/mol的数均分子量（基于H-INMR光谱）和1.17的多分散性。然后研究了这种两亲性三嵌段共聚物的聚集行为。在将聚合物悬浮在四氢呋喃和水的混合物中，随后除去四氢呋喃后，形成球形聚集体。通过应用不同的电子显微镜技术，可以确定这些聚集体是聚合物囊泡，可能与大的化合物胶束共存。(c)2005 Wiley Periodicals，Inc.
The peptide Ac-Ser-Ala-Gly-Ala-Gly-Glu-Gly-Ala-Gly-Ala-Gly-Ser-Gly-OH was prepared with solid-phase peptide chemistry. Before the removal of the peptide from the solid support, the alcohol side groups of the two serines were functionalized with an a-bromo ester moiety to create a bifunctional initiator. This peptide-based initiator was used in solution for the atom transfer radical polymerization of methyl methacrylate to yield a well-defined ABA triblock copolymer, in which the poly(methyl methacrylate) end blocks had a number-average molecular weight of 1.1 kg/mol (based on H-1 NMR spectroscopy) and a polydispersity of 1.17. The aggregation behavior of this amphiphilic triblock copolymer was then investigated. Upon the suspension of the polymer in a mixture of tetrahydrofuran and water, followed by the removal of tetrahydrofuran, spherical aggregates were formed. By the application of different electron microscopy techniques, it was determined that these aggregates were polymersomes, presumably coexisting with large compound micelles. (c) 2005 Wiley Periodicals, Inc.