Leptin induction of UCP1 gene expression is dependent on sympathetic innervation
Leptin induction of UCP1 gene expression is dependent on sympathetic innervation
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DOI：
10.1152/ajpendo.1998.275.2.e259
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发表时间：
1998-08-01
影响因子：
5.1
通讯作者：
Matheny, M
中科院分区：
文献类型：
--
作者：
Scarpace, PJ;Matheny, M
我们以前证明，瘦素增加解偶联蛋白1（UCP 1）和脂蛋白脂酶（LPL）基因表达在棕色脂肪组织（BAT）的大鼠。为了确定这些成绩单的诱导是否依赖于BAT的交感神经支配，我们单方面手术去神经肩胛间BAT在成对喂养和瘦素（0.9毫克/天输注）治疗的大鼠。在成对喂养的大鼠中，去神经BAT垫中的UCP 1 mRNA水平比神经支配的垫低30-47%。在完整的BAT垫，瘦素管理增加近2.5倍的UCP 1的mRNA水平相比，配对喂养的大鼠。相反，在去神经BAT垫，有没有增加UCP 1基因表达。当LPL mRNA在成对喂养的大鼠中进行检查时，神经支配和去神经支配的BAT垫之间没有差异。与瘦素管理，LPL基因表达增加了75%，在神经支配和失神经支配的BAT垫。β 3肾上腺素能受体mRNA不受去神经支配或瘦素的影响，而解偶联蛋白2 mRNA水平在附睾白色脂肪组织（WAT）中增加，但在肾周WAT中不增加。CGP-12177是一种特异性β-肾上腺素能受体激动剂，在神经支配和去神经支配的BAT垫中诱导UCP 1增加近4倍，LPL基因表达增加2倍。这些数据表明，瘦素诱导UCP 1基因表达的BAT是依赖于交感神经支配，但LPL基因表达的瘦素诱导不是。
We previously demonstrated that leptin increases uncoupling protein 1 (UCP1) and lipoprotein lipase (LPL) gene expression in brown adipose tissue (BAT) of rats. To determine whether the induction of these transcripts is dependent on sympathetic innervation of BAT, we unilaterally surgically denervated interscapular BAT in both pair-fed and leptin (0.9 mg/day by infusion)-treated rats. In pair-fed rats, the level of UCP1 mRNA in the denervated BAT pad was 30-47% less than in the innervated pad. In the intact BAT pad, leptin administration increased UCP1 mRNA levels by nearly 2.5-fold compared with pair-fed rats. In contrast, in the denervated BAT pad, there was no increase in UCP1 gene expression. When LPL mRNA was examined in pair-fed rats, there was no difference between innervated and denervated BAT pads. With leptin administration, LPL gene expression increased by 75% in both the innervated and denervated BAT pads. beta(3)-Adrenergic receptor mRNA was unaffected by either denervation or leptin, whereas uncoupling protein 2 mRNA levels were increased in epididymal white adipose tissue (WAT) but not in perirenal WAT. CGP-12177, a specific beta(3)-adrenergic receptor agonist, induced nearly a fourfold increase in UCP1 and a twofold increase in LPL gene expression in both the innervated and denervated BAT pads. These data indicate that the leptin induction of UCP1 gene expression in BAT is dependent on sympathetic innervation but that the leptin induction of LPL gene expression is not.