Chloroplast biogenesis: detection of a magnesium protoporphyrin diester pool in plants.
Chloroplast biogenesis: detection of a magnesium protoporphyrin diester pool in plants.
复制标题
叶绿体生物发生：植物中镁原卟啉二酯库的检测。
DOI：
10.1021/bi00520a039
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Rebeiz,CA
中科院分区：
文献类型：
--
作者：
McCarthy,SA;Belanger,FC;Rebeiz,CA
苏珊·A McCarthy，1 Faith C. Belanger和Constantin A.摘要：在黄化的黄瓜子叶和黑暗生长的纤细裸藻中发现了一种新的金属卟啉池。新的池表现出完全酯化的金属卟啉，没有游离羧基的色谱性能，和镁原卟啉的分光光度和荧光光谱性能。脱金属和水解表明，四吡咯部分的金属卟啉是原卟啉二酯。高压液相色谱的完全酯化的金属卟啉池和气相色谱/质谱分析的皂化醇馏分显示，后者是由三个主要的长链醇。然而，这些醇中没有一种可以与已知的类异戊二烯如香叶醇、法呢醇或植醇鉴别。皂化醇馏分的相似分析
Susan A. McCarthy, 1 Faith C. Belanger, and Constantin A. Rebeiz* abstract: The discovery of a novel metalloporphyrin pool in etiolated cucumber cotyledons and in dark-grown Euglena gracilis is described. The novel pool exhibited the chromatographic properties of a fully esterified metalloporphyrin, devoid of free carboxylic groups, and the spectrophotometric and spectrofluorometric properties of a magnesium protoporphyrin. Demetalation and hydrolysis indicated that the tetrapyrrole moiety of the metalloporphyrin was a protoporphyrin diester. High-pressure liquid chromatography of the fully esterified metalloporphyrin pool and gas chromatographic/mass spectroscopic analysis of the saponified alcohol fraction revealed that the latter was made up of three major long-chain alcohols. None of those alcohols was identifiable, however, with known isoprenoids such as geraniol, farnesol, or phytol. Similar analysis of the saponified alcohol fraction