Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).
Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).
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DOI：
10.1002/jor.22891
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Canty-Laird EG
中科院分区：
文献类型：
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作者：
Williamson KA;Lee KJ;Humphreys WJ;Comerford EJ;Clegg PD;Canty-Laird EG
本研究的目的是表征马趾浅屈肌腱的干细胞和祖细胞群，这是一种能量储存肌腱，与经常受伤的人类跟腱相似。使用已发表的通过低密度平板分离肌腱干细胞/祖细胞的方法，我们发现分离的细胞具有克隆形成性，但使用三系分化测定法无法完全分化为间充质谱系。特别是，通过对脂肪形成培养基中培养的干细胞/祖细胞进行油红O染色评估，脂肪形成分化似乎受到限制。然后，我们评估了是否可以利用对纤连蛋白基质的差异粘附来分离具有更广泛分化潜力的细胞群。然而，我们发现这些细胞的干细胞和肌腱基因表达谱与肌腱细胞相比几乎没有差异，尽管血小板反应蛋白-4 的表达在缺氧条件下显着降低。通过与纤连蛋白的差异粘附分离的肌腱衍生干/祖细胞与通过低密度铺板分离的细胞具有相似的分化潜力，并且在常氧或低氧条件下生长时。总之，尽管有证据表明有干/祖细胞样特征，但我们发现从马指屈肌腱浅层分离的细胞的分化潜力有限。 © 2015 作者。 《骨科研究杂志》由 Wiley periodicals, Inc. 代表骨科研究协会出版。 J Orthop Res 33：849–858，2015。
The aim of this study was to characterize stem and progenitor cell populations from the equine superficial digital flexor tendon, an energy‐storing tendon with similarities to the human Achilles tendon, which is frequently injured. Using published methods for the isolation of tendon‐derived stem/progenitor cells by low‐density plating we found that isolated cells possessed clonogenicity but were unable to fully differentiate towards mesenchymal lineages using trilineage differentiation assays. In particular, adipogenic differentiation appeared to be restricted, as assessed by Oil Red O staining of stem/progenitor cells cultured in adipogenic medium. We then assessed whether differential adhesion to fibronectin substrates could be used to isolate a population of cells with broader differentiation potential. However we found little difference in the stem and tenogenic gene expression profile of these cells as compared to tenocytes, although the expression of thrombospondin‐4 was significantly reduced in hypoxic conditions. Tendon‐derived stem/progenitor cells isolated by differential adhesion to fibronectin had a similar differentiation potential to cells isolated by low density plating, and when grown in either normoxic or hypoxic conditions. In summary, we have found a restricted differentiation potential of cells isolated from the equine superficial digital flexor tendon despite evidence for stem/progenitor‐like characteristics. © 2015 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 33:849–858, 2015.