Expression of src family genes during monocytic differentiation of HL-60 cells.
Expression of src family genes during monocytic differentiation of HL-60 cells.
复制标题
HL-60 细胞单核细胞分化过程中 src 家族基因的表达。
作者：
K. Katagiri;T. Katagiri;Y. Koyama;M. Morikawa;T. Yamamoto;T. Yoshida
据报道，src家族蛋白酪氨酸激酶在造血细胞的某一谱系或分化阶段特异性表达。为了了解单核细胞/巨噬细胞分化和功能的分子基础，我们研究了用分化诱导剂刺激的 HL-60 细胞的 src 家族基因的表达。 TPA 和维生素 D3 (D3) 被用作单核细胞发育的刺激剂，因为已知每种试剂均可诱导 HL-60 细胞的表型特异性分化。 fyn、fgr 和 lyn 基因的特征性表达伴随着表型变化和核原癌基因的表达，而 src、lck、hck 和 yes 基因则不然。在TPA诱导的HL-60细胞分化中，fyn和lyn基因均表达，但fgr基因不表达。相反，在D3诱导的细胞分化中，fgr和lyn基因均表达，但fyn基因不表达。在TPA加D3或D3加人转化生长因子-β1诱导的HL-60细胞的进一步高级分化中观察到这些基因的独立和特征性表达。维甲酸处理的细胞的粒细胞分化伴随着fgr的强烈表达，而lyn和fyn基因的表达较弱或不表达。这些数据表明，src家族基因编码的每种蛋白酪氨酸激酶可能在单核细胞/巨噬细胞谱系细胞的发育和/或功能中发挥不同的作用。
It has been reported that src family protein-tyrosine kinases were expressed specifically in a certain lineage or differentiation stage of hematopoietic cells. To understand the molecular basis for differentiation and function of monocyte/macrophage, we investigated the expressions of src family genes by the HL-60 cells stimulated with differentiation-inducing agents. TPA and vitamin D3 (D3) were used as stimulants for monocytic development, since each agent has been known to induce phenotypically specific differentiation of HL-60 cells. The fyn, fgr, and lyn genes were characteristically expressed concomitantly with phenotypic changes and expressions of nuclear proto-oncogenes, whereas src, lck, hck, and yes genes were not. In TPA-induced differentiation of HL-60 cells, both fyn and lyn genes, but not fgr gene, were expressed. In contrast, both fgr and lyn genes, but not fyn gene, were expressed in D3-induced differentiation of the cells. The independent and characteristic expressions of these genes were observed in the further advanced differentiation of HL-60 cells induced by TPA plus D3 or D3 plus human transforming growth factor-beta 1. The granulocytic differentiation of the cells treated with retinoic acid was accompanied by intense expression of fgr, but weak or no expression of lyn and fyn gene. These data indicate that each protein-tyrosine kinase encoded by src family genes may play distinct roles in development and/or functions of monocyte/macrophage-lineage cells.