Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei
Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei
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DOI：
10.1104/pp.107.115246
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发表时间：
2008-05-01
期刊：
影响因子：
7.4
通讯作者：
Galbraith, David W.
中科院分区：
文献类型：
--
作者：
Zhang, Changqing;Barthelson, Roger A.;Galbraith, David W.
我们描述了一种简单而高效的方法，用于在复杂组织内的特定细胞类型内表达的基因的全局识别。它涉及以细胞类型特异性方式转基因表达核靶向的绿色荧光蛋白。然后通过荧光激活分选从匀浆中纯化荧光细胞核，并将RNA用作微阵列杂交的靶。我们证明了该方法的有效性，通过鉴定12个基因，选择性地表达在韧皮部。
We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.