The vagus nerve is one route of transneural invasion for intranasally inoculated influenza A virus in mice
The vagus nerve is one route of transneural invasion for intranasally inoculated influenza A virus in mice
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DOI：
10.1354/vp.41-2-101
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发表时间：
2004-03-01
影响因子：
2.4
通讯作者：
Umemura, T
中科院分区：
文献类型：
--
作者：
Matsuda, K;Park, CH;Umemura, T
鼻内接种的亲神经性流感病毒在小鼠中不仅感染呼吸道，而且感染中枢神经系统（CNS），主要是脑干。(26以往的研究表明病毒侵入中枢神经系统的途径是通过周围神经系统，尤其是迷走神经。为了评估该病毒的经迷走神经传播，我们用强毒株流感病毒（24a5b株）鼻内接种单侧迷走神经切除小鼠，并通过免疫组织化学和原位杂交随时间推移检查病毒蛋白和基因组的分布。在迷走神经（结状）神经节之间观察到病毒抗原的不对称分布：在迷走神经切除侧的迷走神经节中检测到病毒抗原比在完整侧的迷走神经节中晚2天。病毒从呼吸道粘膜经迷走神经直接交叉转运至中枢神经系统，并经迷走神经节离心转运至迷走神经节。因此，本研究的结果表明，嗜神经性流感病毒主要通过迷走神经传播到CNS。
Intranasally inoculated neurotropic influenza viruses in mice infect not only the respiratory tract but also the central nervous system (CNS), mainly the brain stem.(26,34) Previous studies suggested that the route of invasion of virus into the CNS was via the peripheral nervous system, especially the vagus nerve. To evaluate the transvagal transmission of the virus, we intranasally inoculated unilaterally vagectomized mice with a virulent influenza virus (strain 24a5b) and examined the distribution of the viral protein and genome by immunohistochemistry and in situ hybridization over time. An asymmetric distribution of viral antigens was observed between vagal (nodose) ganglia: viral antigen was detected in the vagal ganglion of the vagectomized side 2 days later than in the vagal ganglion of the intact side. The virus was apparently transported from the respiratory mucosa to the CNS directly and decussately via the vagus nerve and centrifugally to the vagal ganglion of the vagectomized side. The results of this study, thus, demonstrate that neurotropic influenza virus,:,an, travels to the CNS mainly via the vagus nerve.