Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy
Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy
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DOI：
10.1093/jmicro/dfs001
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发表时间：
2012-04-01
期刊：
影响因子：
--
通讯作者：
Rhyu, Im Joo
中科院分区：
文献类型：
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作者：
Kim, Hyun-Wook;Oh, Seung Hak;Rhyu, Im Joo
真核生物中的线粒体是重要的细胞器，负责三磷酸腺苷的合成，钙稳态和类固醇合成。由于线粒体的结构和分布取决于其功能和细胞条件而高度多样化，因此开发快速准确的方法来评估其形态非常重要。在这项研究中，我们使用高压电子显微镜（HVEM）可视化培养细胞中的整个线粒体。与传统的透射电子显微镜方法相比，本方法不需要薄切片，因此需要更少的时间进行图像采集和处理。此外，与基于荧光的光学显微镜方法相比，我们的方法提供了更准确的尺寸信息。因此，我们建议HVEM是一个有用的工具，快速，准确地分析线粒体的形态和分布在细胞中。
Mitochondria in all eukaryotes are essential organelles responsible for adenosine triphosphate synthesis, calcium homeostasis and steroidogenesis. Because the structure and distribution of mitochondria are highly diverse depending on their function and cellular conditions, it is important to develop a rapid and accurate method to assess their morphology. In this study, we visualize whole mitochondria in cultured cells using high-voltage electron microscopy (HVEM). Compared with conventional transmission electron microscopic approaches, the present method does not require thin sectioning and thus requires less time for image acquisition and processing. Furthermore, compared with fluorescence-based light microscopic approaches, our method provides more accurate size information. Thus, we propose that HVEM is a useful tool for rapid and accurate analysis of mitochondrial morphology and distribution in a cell.