Experimental evaluation of anti-inflammatory effect of topical application of entada phaseoloides seeds as paste and ointment.
Experimental evaluation of anti-inflammatory effect of topical application of entada phaseoloides seeds as paste and ointment.
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DOI：
10.4297/najms.2011.3513
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Rajopadhye BD
中科院分区：
文献类型：
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作者：
Dawane JS;Pandit VA;Rajopadhye BD
本研究旨在研究不同剂型的鸡骨草种子浆局部应用的抗炎作用。在去除壳后，将紫豆种子研磨成粉。用水制备糊剂，用聚乙二醇和Carbowax 3350制备软膏。32只体重140-200 g的Wister大鼠，雌雄不限，分为4组，I组为赋形剂组，II组为恩他达-四季青糊剂组，III组为恩他达-四季青软膏组，IV组为双氯芬酸钠软膏组。通过在左后爪的足底下区域注射0.1ml完全弗氏佐剂来诱导关节炎。当天开始药物治疗，持续12天。在第0、1、5、12和21天，用Plethysmometer测量两只爪的爪体积。在整个研究期间观察体重和步态。24 h内所有大鼠均出现局部炎症反应。对照组21天内肿胀仍未消退。与对照相比，两种EP制剂均显示出显著（P < 0.001）的抗炎活性。第12天，与双氯芬酸钠疗效相当。第21天，白豆糊剂的疗效显著优于双氯芬酸钠（P < 0.05）。两种剂型的恩他达均具有抗炎活性，恩他达糊剂的抗炎活性明显优于双氯芬酸钠。
The study was to study the anti-inflammatory effect of topical application of different formulations of seed pulp of entada phaseoloides. After removing the shell, entada phaseoloides seeds were powdered. Paste was prepared with water and ointment with polyethylene glycol & Carbowax 3350. 32 Wister rats of either sex weighing 140-200 gram were divided into four groups, Group-I vehicle, Group-II entada phaseoloides paste, Group-III entada phaseoloides Ointment, Group-IV Diclofenac sodium Ointment. Arthritis was induced by injecting 0.1ml Complete Freund's adjuvant in sub plantar region of the left hind paw. Drug treatment was started on the same day and given for 12 days. Paw volume was measured with Plethysmometer on day 0, 1, 5, 12 and 21 for both the paws. Bodyweight and Gait was observed throughout the study. Localized inflammatory reaction developed in all the rats in 24 hours. In control group, there was no resolution of swelling even in 21 days. Both EP formulations showed significant (P < 0.001) anti-inflammatory activity as compared to control. entada phaseoloides ointment was equi-effective to that of Diclofenac sodium on 12th day. entada phaseoloides paste was significantly (P < 0.05) more effective than Diclofenac sodium on 21st day. Both the formulations of entada phaseoloides have anti-inflammatory activity and entada phaseoloides paste is significantly more effective than diclofenac sodium.