Electron nuclear double resonance of semiquinones in reaction centers of Rhodopseudomonas sphaeroides.
Electron nuclear double resonance of semiquinones in reaction centers of Rhodopseudomonas sphaeroides.
复制标题
球形红假单胞菌反应中心半醌的电子核双共振。
DOI：
10.1016/0005-2728(85)90155-0
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Feher,G
中科院分区：
文献类型：
--
作者：
Lubitz,W;Abresch,EC;Debus,RJ;Isaacson,RA;Okamura,MY;Feher,G
用Zn ~（2+）置换红球藻反应中心的Fe ~（2+），使我们能够对一级和二级泛醌受体Q-A和Q-B的阴离子自由基进行ENDOR（电子核双共振）实验。通过对ENDOR光谱的分析，我们推断出了QA和QB位点之间的差异、与氧的氢键、与蛋白质质子的相互作用以及结合位点的一些对称性。
Replacement of Fe2+by Zn2+in reaction centers ofRhodopseudomonas sphaeroidesenabled us to perform ENDOR (electron nuclear double resonance) experiments on the anion radicals of the primary and secondary ubiquinone acceptors Q−Aand Q−B. Differences between the QAand QBsites, hydrogen bonding to the oxygens, interactions with the protons of the proteins and some symmetry properties of the binding sites were deduced from an analysis of the ENDOR spectra.