MONOCLONAL-ANTIBODIES IDENTIFY FC-GAMMA RECEPTORS ON UNFERTILIZED HUMAN OOCYTES BUT NOT SPERMATOZOA
MONOCLONAL-ANTIBODIES IDENTIFY FC-GAMMA RECEPTORS ON UNFERTILIZED HUMAN OOCYTES BUT NOT SPERMATOZOA
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DOI：
10.1016/0165-0378(92)90032-y
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发表时间：
1992-04-01
影响因子：
3.4
通讯作者：
FLEIT, HB
中科院分区：
文献类型：
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作者：
BRONSON, RA;FUSI, FM;FLEIT, HB
卵膜Fc受体先前已被证明在促进抗体标记的人精子与无透明带仓鼠卵的粘附中起作用。在这项工作中，我们证明了存在的Fc-γ-RI，Fc-γ-RII和Fc-γ-RIII的卵膜上的未受精的人卵母细胞通过针对这些受体的单克隆抗体，检测免疫珠玫瑰花结和间接免疫荧光。这些受体也具有功能活性，因为它们能够结合人聚集IgG、人IgG-Fc、小鼠IgG 1和IgG 2a。尽管卵膜IgG-Fc受体的存在可能通过促进多精子受精而在生殖失败中发挥作用，但在抗精子抗体结合精子表面的情况下，其在正常受精生理学或与精子结合无关的其他事件中的作用仍有待确定。相比之下，Fc-γ受体不存在于人精子上，无论其功能状态如何（新鲜射精、获能或顶体反应）。
Oolemmal Fc receptors have previously been shown to play a role in the promotion of adhesion by antibody labeled human spermatozoa to zona-free hamster eggs. In this work, we demonstrated the presence of Fc-gamma-RI, Fc-gamma-RII and Fc-gamma-RIII on the oolemma of unfertilized human oocytes by means of monoclonal antibodies directed against these receptors, detected both by immunobead rosetting and indirect immunofluorescence. These receptors were also functionally active in that they were able to bind human aggregated IgG, human IgG-Fc, mouse IgG1 and IgG2a. While the presence of oolemmal IgG-Fc receptors might play a role in reproductive failure, by their promotion of polyspermic fertilization, in cases where antisperm antibodies bound to the spermatozoan surface, their role in the normal physiology of fertilization or in other events unrelated to sperm incorporation remains to be determined. In contrast, Fc-gamma-receptors were not present on human spermatozoa, irrespective of their functional state (fresh ejaculated, capacitated or acrosome reacted).