Mast cells in the rat brain synthesize gonadotropin-releasing hormone
Mast cells in the rat brain synthesize gonadotropin-releasing hormone
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DOI：
10.1002/neu.10220
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发表时间：
2003-08-01
期刊：
影响因子：
--
通讯作者：
Silver, R
中科院分区：
文献类型：
--
作者：
Khalil, MH;Silverman, AJ;Silver, R
肥大细胞存在于大脑中，它们的数量随着生殖状态的变化而变化。虽然有人认为脑肥大细胞含有哺乳动物下丘脑形式的促性腺激素释放激素(GnRH-I)，但肥大细胞是否从头合成GnRH-I尚不清楚。在本研究中，大鼠丘脑中的肥大细胞对GnRH-I和GnRH-I相关肽(GAP)产生的抗血清呈免疫反应；肥大细胞的特异性分子肝素或5-羟色胺的存在证实了肥大细胞的身份。为了检测肥大细胞是否合成GnRH-I mRNA，用GnRH-I cRNA探针进行原位杂交，通过亲和素与肝素的结合鉴定信号存在于肥大细胞内。从腹膜腔分离的肥大细胞中也检测到GnRH-I基因的表达。鉴于GnRH-I在生殖调节中的作用，我们研究了脑内GnRH-I肥大细胞数量的变化。当雄性动物与性欲强的雌性动物共住2小时或5天时，脑部肥大细胞的数量显著增加，而GnRH-I阳性的肥大细胞比例与与熟悉的雄性动物共处的雄性动物相似。这些发现是第一个表明肥大细胞合成GnRH-I的报道，在生殖环境中出现的肥大细胞增加是GnRH-I阳性和非GnRH-I阳性肥大细胞平行增加的结果。(C)2003年威利期刊公司。
Mast cells occur in the brain and their number changes with reproductive status. While it has been suggested that brain mast cells contain the mammalian hypothalamic form of gonadotropin-releasing hormone (GnRH-I), it is not known whether mast cells synthesize GnRH-I de novo. In the present study, mast cells in the rat thalamus were immunoreactive to antisera generated against GnRH-I and the GnRH-I associated peptide (GAP); mast cell identity was confirmed by the presence of heparin, a molecule specific to mast cells, or serotonin. To test whether mast cells synthesize GnRH-I mRNA, in situ hybridization was performed using a GnRH-I cRNA probe, and the signal was identified as being within mast cells by the binding of avidin to heparin. GnRH-I mRNA was also found, using RT-PCR, in mast cells isolated from the peritoneal cavity. Given the function of GnRH-I in the regulation of reproduction, changes in the population of brain GnRH-I mast cells were investigated. While housing males with sexually receptive females for 2 h or 5 days resulted in a significant increase in the number of brain mast cells, the proportion of mast cells positive for GnRH-I was similar to that in males housed with a familiar male. These findings represent the first report showing that mast cells synthesize GnRH-I and that the mast cell increase seen in a reproductive context is the result of a parallel increase in GnRH-I positive and non-GnRH-I positive mast cells. (C) 2003 Wiley Periodicals, Inc.