Bioethanol production from barley hull using SAA (soaking in aqueous ammonia) pretreatment
Bioethanol production from barley hull using SAA (soaking in aqueous ammonia) pretreatment
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DOI：
10.1016/j.biortech.2007.10.055
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发表时间：
2008-09-01
影响因子：
11.4
通讯作者：
Hicks, Kevin B.
中科院分区：
文献类型：
--
作者：
Kim, Tae Hyun;Taylor, Frank;Hicks, Kevin B.
大麦船体是一种木质纤维素类生物质，采用氨水预处理，将其转化为乙醇。将大麦船体浸泡在15和30重量%的在30、60和75摄氏度的氨水中持续12小时至11周。这种预处理方法被称为“氨水浸泡”（SAA）。在试验条件中，观察到的最佳预处理条件为75 ℃，48 h，15 wt.%氨水和1：12的固液比，导致葡聚糖的糖化产率为83%，木聚糖的糖化产率为63%，具有15 FPU/g-葡聚糖酶负载。使用15 wt.%在75 ℃下用氨处理24-72小时，从固体中除去50-66%的原始木质素，同时保留65-76%的木聚糖而没有任何葡聚糖损失。添加木聚糖酶沿着纤维素酶对SAA处理的大麦船体和重组E. coli（KO11）。在3%w/v葡聚糖加载量和4 mL木聚糖酶加载量的情况下，SAA处理的大麦船体的SSCF在15 FPU纤维素酶/g-葡聚糖加载量下产生24.1g/L乙醇浓度，其对应于基于葡聚糖和木聚糖的最大理论产率的89.4%。SEM结果表明，SAA处理增加了比表面积和孔径。据推测，这些物理变化提高了SAA处理的大麦船体的酶消化率。(C)2007爱思唯尔有限公司保留所有权利。
Barley hull, a lignocellulosic biomass, was pretreated using aqueous ammonia, to be converted into ethanol. Barley hull was soaked in 15 and 30 wt.% aqueous ammonia at 30, 60, and 75 degrees C for between 12 h and 11 weeks. This pretreatment method has been known as "soaking in aqueous ammonia" (SAA). Among the tested conditions, the best pretreatment conditions observed were 75 degrees C, 48 h, 15 wt.% aqueous ammonia and 1:12 of solid:liquid ratio resulting in saccharification yields of 83% for glucan and 63% for xylan with 15 FPU/g-glucan enzyme loading. Pretreatment using 15 wt.% ammonia for 24-72 h at 75 degrees C removed 50-66% of the original lignin from the solids while it retained 65-76% of the xylan without any glucan loss. Addition of xylanase along with cellulase resulted in synergetic effect on ethanol production in SSCF (simultaneous saccharification and co-fermentation) using SAA-treated barley hull and recombinant E. coli (KO11). With 3% w/v glucan loading and 4 mL of xylanase enzyme loadings, the SSCF of the SAA treated barley hull resulted 24.1 g/L ethanol concentration at 15 FPU cellulase/g-glucan loading, which corresponds to 89.4% of the maximum theoretical yield based on glucan and xylan. SEM results indicated that SAA treatment increased surface area and the pore size. It is postulated that these physical changes enhance the enzymatic digestibility in the SAA treated barley hull. (C) 2007 Elsevier Ltd. All rights reserved.