Fouling-Resistant Hydrogels Prepared by the Swelling-Assisted Infusion and Polymerization of Dopamine
Fouling-Resistant Hydrogels Prepared by the Swelling-Assisted Infusion and Polymerization of Dopamine
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DOI：
10.1021/acsabm.8b00001
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发表时间：
2018-07-16
影响因子：
4.7
通讯作者：
Schiffman, Jessica D.
中科院分区：
文献类型：
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作者：
Kolewe, Kristopher W.;Dobosz, Kerianne M.;Schiffman, Jessica D.
由于抗生素耐药性的蔓延，医疗设备引起的生物膜相关感染越来越具有挑战性。在这项研究中，我们提出了一种简单的策略，通过掺入抗污聚合物两性离子聚（2-甲基丙烯酰氧乙基磷酸胆碱）（pMPC），显著提高共价交联聚乙二醇（PEG）和物理交联琼脂水凝胶的防污性能。在水凝胶膨胀过程中，多巴胺聚合被启动，这提供了多巴胺和pMPC，一种渗透驱动力进入水凝胶内部。聚乙二醇和琼脂水凝胶的储存模量（1.7-1300 kPa）范围很广，经pMPC和聚多巴胺（PDA）功能化后，两者在统计学上保持相等。当用纤维蛋白原（一种模型凝血蛋白）刺激时，pMPC/ pda功能化的PEG和琼脂水凝胶显示，与水凝胶对照相比，蛋白质吸附减少了约90%。此外，观察到大肠杆菌和金黄色葡萄球菌的粘附性降低了一个数量级以上。本研究展示了一个多功能材料平台，通过独立于原始水凝胶的化学成分和网络结构的pMPC/PDA掺入策略来增强水凝胶的抗污性。
Biofilm-associated infections stemming from medical devices are increasingly challenging to treat due to the spread of antibiotic resistance. In this study, we present a simple strategy that significantly enhances the antifouling performance of covalently cross-linked poly(ethylene glycol) (PEG) and physically cross-linked agar hydrogels by incorporation of the fouling-resistant polymer zwitterion, poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC). Dopamine polymerization was initiated during swelling of the hydrogels, which provided dopamine and pMPC, an osmotic driving force into the hydrogel interior. Both PEG and agar hydrogels were synthesized over a broad range of storage moduli (1.7-1300 kPa), which remained statistically equivalent after being functionalized with pMPC and polydopamine (PDA). When challenged with fibrinogen, a model blood-clotting protein, the pMPC/PDA-functionalized PEG and agar hydrogels displayed a >90% reduction in protein adsorption compared to hydrogel controls. Further, greater than an order-of-magnitude reduction in Escherichia coli and Staphylococcus aureus adherence was observed. This study demonstrates a versatile material platform to enhance the fouling resistance of hydrogels through a pMPC/PDA incorporation strategy that is independent of the chemical composition and network structure of the original hydrogel.