latheo, a new gene involved in associative learning and memory in Drosophila melanogaster, identified from P element mutagenesis.
latheo, a new gene involved in associative learning and memory in Drosophila melanogaster, identified from P element mutagenesis.
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latheo 是从 P 元件诱变中鉴定出的黑腹果蝇中涉及联想学习和记忆的新基因。
DOI：
10.1093/genetics/131.3.655
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Tully,T
中科院分区：
文献类型：
--
作者：
Boynton,S;Tully,T
由于甲基磺酸乙酯（EMS）诱导的突变只存在于少数X连锁基因中，果蝇学习和记忆的遗传解剖受到限制。为了弥补这一缺陷，我们已经开始了P元件诱变筛选常染色体突变，破坏联想学习和/或记忆。“P标记”突变等位基因的产生将加快这些新基因的分子克隆。在这里，我们描述了一个行为遗传特征的latheoP 1，一个隐性的，亚形突变的一个必要的基因。latheoP 1果蝇在嗅觉回避条件反射实验中表现不佳。这种表现缺陷不能归因于异常的嗅觉敏锐度或休克反应-两个任务相关的“周边”行为，这是在经典的条件反射。因此，latheoP 1突变似乎特别影响学习/记忆。与P元件插入的染色体原位定位一致，第二条染色体的49 F区域的缺陷未能补充latheoP 1突变的行为效应。进一步的互补分析latheoP 1和致死等位基因，产生的latheoP 1插入或EMS或γ-射线切除，在49 F区域映射的latheo突变的一个重要的互补组。latheoP 1和两个EMS致死等位基因之一或一个致死切除等位基因杂合的果蝇也显示出行为缺陷，从而证明行为和致死表型共映射到相同的基因座。
Genetic dissection of learning and memory in Drosophila has been limited by the existence of ethyl methanesulfonate (EMS)-induced mutations in only a small number of X-linked genes. To remedy this shortcoming, we have begun a P element mutagenesis to screen for autosomal mutations that disrupt associative learning and/or memory. The generation of "P-tagged" mutant alleles will expedite molecular cloning of these new genes. Here, we describe a behavior-genetic characterization of latheoP1, a recessive, hypomorphic mutation of an essential gene. latheoP1 flies perform poorly in olfactory avoidance conditioning experiments. This performance deficit could not be attributed to abnormal olfactory acuity or shock reactivity-two task-relevant "peripheral" behaviors which are used during classical conditioning. Thus, the latheoP1 mutation appears to affect learning/memory specifically. Consistent with chromosomal in situ localization of the P element insertion, deficiencies of the 49F region of the second chromosome failed to complement the behavioral effect of the latheoP1 mutation. Further complementation analyses between latheoP1 and lethal alleles, produced by excision of the latheoP1 insert or by EMS or gamma-rays, in the 49F region mapped the latheo mutation to one vital complementation group. Flies heterozygous for latheoP1 and one of two EMS lethal alleles or one lethal excision allele also show the behavioral deficits, thereby demonstrating that the behavioral and lethal phenotypes co-map to the same locus.