On Baecchi’s staining method with reference to Nakagawa et al. (2021): “Advantages of filtration method for sperm-DNA genotyping in sexual assault cases”
On Baecchi’s staining method with reference to Nakagawa et al. (2021): “Advantages of filtration method for sperm-DNA genotyping in sexual assault cases”
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关于 Baecchi 的染色方法，参考 Nakakawa 等人 (2021)：“性侵犯案件中精子 DNA 基因分型的过滤方法的优点”
DOI：
10.1016/j.legalmed.2022.102176
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发表时间：
2023
期刊：
影响因子：
1.5
通讯作者：
Sonoda A
中科院分区：
文献类型：
--
作者：
Yukawa N;Shinkawa N;Yano K;Tachibana M;Kakizaki E;Sonoda A
在最近的一篇文章中，Nakagawa et al. [1]开发了一种新的过滤方法，使用1：1：40的1%酸性品红、1%亚甲蓝和1 M HCl溶液（他们称之为Baecchi染色液）从法医样品中分离精子用于精子DNA基因分型。1912年，意大利法医科学家Baecchi用德语写了一篇关于使用甲基蓝作为蓝色染料的广泛工作[2]，同时否认使用亚甲蓝，这篇工作被认为是最近研究中这种方法的权威文章[3-6]。虽然亚甲蓝和甲基蓝听起来相似，但它们是化学上不同的物质（图1-A，1-B）[7]。因此，Nakagawa et al. [1]不是Baecchi染色液，应更精确地描述（例如，用亚甲蓝代替甲基蓝的改良Baecchi染色液）。为了进一步阐明Baecchi染色液的命名和组成，应该指出的是，Baecchi最初在1909年报告了亚甲蓝的使用[8]。并且日本的其他报告和教科书将Baecchi的名字归因于使用1：1：1的亚甲蓝的双重染色溶液或方法。40 1%酸性品红、1%亚甲蓝和1% HCl溶液（约0.28 M，比重为1.02）[9，10]，其组成为0.024%酸性品红、0.024%亚甲蓝和0.95% HCl [11，12]，还涉及在没有特定配方或组合物的情况下使用酸性品红和亚甲基蓝[13，14]。在最初的报告[8]中，
In a recent article, Nakagawa et al.[1] developed a novel filtration method to isolate sperm from forensic samples for sperm-DNA genotyping using a 1: 1: 40 solution of 1% acid fuchsin, 1% methylene blue, and 1 M HCl which they called Baecchi’s staining solution. In 1912, Baecchi, an Italian forensic scientist, wrote an extensive work in German [2] regarding the use of methyl blue as a blue dye while denying the use of methylene blue, and this work is regarded as the authoritative article for this method in more recent studies [3-6]. While methylene blue and methyl blue sound similar, they are chemically distinct substances (Fig. 1-A, 1-B)[7]. Thus, the solution described in Nakagawa et al.[1] is not Baecchi’s staining solution and should have been described more precisely (eg, modified Baecchi’s staining solution with methyl blue replaced by methylene blue).To further clarify the naming and composition of Baecchi’s staining solution, it should be stated that Baecchi initially reported the use of methylene blue in 1909 [8] and that other reports and textbooks in Japan have attributed Baecchi’s name to a double staining solution (s) or method (s) using methylene blue in a 1: 1: 40 solution of 1% acid fuchsin, 1% methylene blue, and 1% HCl (about 0.28 M with specific gravity of 1.02)[9, 10], which has a composition of 0.024% acid fuchsin, 0.024% methylene blue and 0.95% HCl [11, 12], and also to the use of acid fuchsin and methylene blue without a specified recipe or composition [13, 14]. In the initial report [8], double staining of