Reduced sialylation of podocalyxin--the major sialoprotein of the rat kidney glomerulus--in aminonucleoside nephrosis.
Reduced sialylation of podocalyxin--the major sialoprotein of the rat kidney glomerulus--in aminonucleoside nephrosis.
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发表时间：
1985-03
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通讯作者：
D. Kerjaschki;A. Vernillo;M. Farquhar
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作者：
D. Kerjaschki;A. Vernillo;M. Farquhar
本研究测定了氨基核苷嘌呤处理(PAN)大鼠和正常对照大鼠足月桂糖苷的糖组成。正常大鼠和PAN大鼠的Podocalyin与硝酸纤维素转移上的125I-WGA和125I-花生凝集素(后者仅在神经氨酸酶处理后)结合。气-液色谱分析表明，从正常大鼠和PAN大鼠中提取的足叶菜素含有唾液酸、半乳糖、GlcNAc和Man，但不含Fuc和GalNAc。在PAN大鼠，其唾液酸含量从4.5%降至1.5%，而其他糖的浓度(可能除半乳糖外)与对照组相似。免疫金标记法测定细胞表面的粘附素密度，PAN大鼠与正常对照组比较无明显差异。这些数据表明，在PAN中发现的肾小球总唾液酸含量的减少是由于足细胞质膜表面积减少和足细胞胞浆唾液酸含量减少的共同作用。
In this study the sugar composition of podocalyxin was determined in puromycin aminonucleoside-treated (PAN) rats and controls. Podocalyxin from both control and PAN rats bound 125I-WGA and 125I-peanut lectin (the latter only after neuraminidase treatment) on nitrocellulose transfers. Purified podocalyxin from both control and PAN rats was found to contain sialic acid, Gal, GlcNac, and Man but lacked Fuc and GalNac by gas-liquid chromatography. In PAN rats the sialic acid content of podocalyxin was reduced from 4.5% to 1.5%, whereas the concentration of the other sugars (with the possible exception of Gal) was similar to that of controls. The density of podocalyxin on the epithelial cell surface was estimated after immunogold labeling with anti-podocalyxin IgG, and no differences were found between PAN rats and controls. These data indicate that the reduced total glomerular sialic acid content found in PAN is due to the combined effects of the decreased podocyte plasmalemmal surface area and the reduced sialic acid content of podocalyxin.