Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate
Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate
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DOI：
10.1073/pnas.122231199
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发表时间：
2002-06
影响因子：
11.1
通讯作者：
M. Zirlinger;Liching Lo;J. McMahon;A. McMahon;D. Anderson
中科院分区：
文献类型：
--
作者：
M. Zirlinger;Liching Lo;J. McMahon;A. McMahon;D. Anderson
谱系追踪实验表明，一些前迁移神经嵴细胞（NCC）是多能的，产生感觉和交感神经元及其相关的胶质细胞。使用诱导型Cre重组酶为基础的命运映射系统，我们已经永久标记的NCC亚群，表达Ngn2，感觉神经发生所需的bHLH转录因子，并比较其命运的散装NCC人口标记的Wnt1的表达。Ngn 2+祖细胞比Wnt 1 + NCC贡献感觉神经节而不是交感神经节的可能性高4倍。然而，在背根神经节内，Ngn 2和Wnt 1表达细胞同样可能产生神经元或神经胶质。这些数据表明，Ngn 2标志着NCC亚群在迁移早期具有可预测的命运偏差。与以前的工作一起，这些数据表明，NCC在成为神经元或神经胶质细胞的命运之前被限制在感觉或自主神经亚系。
Lineage-tracing experiments have indicated that some premigratory neural crest cells (NCCs) are pleuripotent, generating sensory and sympathetic neurons and their associated glia. Using an inducible Cre recombinase-based fate mapping system, we have permanently marked a subpopulation of NCCs that expresses Ngn2, a bHLH transcription factor required for sensory neurogenesis, and compared its fate to the bulk NCC population marked by expression of Wnt1. Ngn2+ progenitors were four times more likely than Wnt1+ NCCs to contribute to sensory rather than sympathetic ganglia. Within dorsal root ganglia, however, both Ngn2- and Wnt1-expressing cells were equally likely to generate neurons or glia. These data suggest that Ngn2 marks an NCC subpopulation with a predictable fate bias, early in migration. Taken together with previous work, these data suggest that NCCs become restricted to sensory or autonomic sublineages before becoming committed to neuronal or glial fates.