Identification of volatile compounds used in host location by the black bean aphid, Aphis fabae
Identification of volatile compounds used in host location by the black bean aphid, Aphis fabae
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DOI：
10.1007/s10886-008-9510-7
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发表时间：
2008-09-01
影响因子：
2.3
通讯作者：
Pickett, John
中科院分区：
文献类型：
--
作者：
Webster, Ben;Bruce, Toby;Pickett, John
蚕豆蚜对蚕豆挥发物的行为和电生理反应。萨顿矮），植物进行了研究，并确定了用于寄主定位的信息化学物质。在嗅觉仪生物测定中，蚜虫在存在完整植株的蚕豆挥发物的嗅觉仪区域比在具有清洁空气的对照区域花费显著更多的时间。当蚕豆植物的空气夹带样品用作气味源时，也发生这种反应。耦合气相色谱-electroantennography揭示了16电生理活性化合物的存在下，在空气夹带样品。其中15种被鉴定为（Z）-3-己烯-1-醇、1-己醇、（E）-2-己烯醛、苯甲醛、6-甲基-5-庚烯-2-酮、辛醛、（Z）-3-己烯-1-基乙酸酯、（R）-（-）-芳樟醇、水杨酸甲酯、癸醛、十一醛、（E）-香芹烯、（E）-β-法呢烯、（S）-（-）-大根香叶烯D和（E，E，）-4，8，12-三甲基-1，3，7，11-十三碳四烯观察到嗅觉计响应于15种组分的合成共混物，所述合成共混物包括与天然样品中相同浓度和比例的所有鉴定的化合物，蚜虫在嗅觉计的处理区域中花费的时间显著更多，其中挥发物存在于对照区域中。这些数据进行了讨论的背景下，昆虫寄主的位置和作物保护。
Behavioral and electrophysiological responses of winged Aphis fabae to volatiles of faba bean, Vicia faba (var. Sutton dwarf), plants were studied and semiochemicals used in host location were identified. In olfactometer bioassays, aphids spent significantly more time in the region of the olfactometer where V. faba volatiles from an intact plant were present than in control regions with clean air. This response also occurred when an air entrainment sample of a V. faba plant was used as the odor source. Coupled gas chromatography-electroantennography revealed the presence of 16 electrophysiologically active compounds in the air entrainment sample. Fifteen of these were identified as (Z)-3-hexen-1-ol, 1-hexanol, (E)-2-hexenal, benzaldehyde, 6-methyl-5-hepten-2-one, octanal, (Z)-3-hexen-1-yl acetate, (R)-(-)-linalool, methyl salicylate, decanal, undecanal, (E)-caryophyllene, (E)-beta-farnesene, (S)-(-)-germacrene D, and (E,E,)-4,8,12-trimethyl-1,3,7,11-tridecatetraene. An olfactometer response was observed to a 15-component synthetic blend that comprised all identified compounds at the same concentration and ratio as in the natural sample, with the aphids spending significantly more time in the treated regions of the olfactometer where volatiles were present than in the control regions. These data are discussed in the context of insect host location and crop protection.