Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies
Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies
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DOI：
10.1007/s10096-010-0912-4
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Greub, G.
中科院分区：
文献类型：
--
作者：
Baud, D.;Regan, L.;Greub, G.
沙眼衣原体特异性抗体的检测对反复流产、输卵管性不孕及宫外孕的诊断有重要价值。我们在此比较了免疫荧光（IF）与其他四种商业检测抗C.沙眼：分别由Medac和R-Biopharm（RB）商业化的两种使用主要外膜蛋白（MOMP）作为抗原的酶联免疫吸附测定（ELISA），一种使用衣原体热休克蛋白60（cHSP 60）作为抗原的ELISA（Medac），以及一种新的自动荧光免疫测定（InoDiag）。共有405例患者（n = 251）和没有（n = 154）流产进行了测试的所有五个测试。C.通过IF、cHSP 60-Medac、MOMP-Medac、MOMP-RB和InoDiag测定的沙眼特异性IgG抗体的阳性率分别为14.3、23.2、14.3、11.9和26.2%。InoDiag显示出最高的灵敏度，而MOMP-RB显示出最好的特异性。与C.使用IF、MOMP-RB和InoDiag检测肺炎衣原体，使用cHSP 60 ELISA检测棘阿米巴副衣原体。C.沙眼衣原体和其它衣原体目（哈特曼新衣原体、Waddlia chondrophila和Simkania negevensis）。鉴于其高灵敏度，InoDiag的新型自动荧光免疫分析是一种有趣的替代方案。R-Biopharm的基于MOMP的ELISA由于其高通量和优异的特异性，应优选用于大型血清学研究。
Screening for Chlamydia trachomatis-specific antibodies is valuable in investigating recurrent miscarriage, tubal infertility and extrauterine pregnancy. We compared here the performance of immunofluorescence (IF) to four other commercial tests in detecting IgG antibodies directed against C. trachomatis: two enzyme-linked immunosorbent assays (ELISAs) using the major outer membrane protein (MOMP) as the antigen, commercialised respectively by Medac and R-Biopharm (RB), one ELISA using the chlamydial heat shock protein 60 (cHSP60) as the antigen (Medac), as well as a new automated epifluorescence immunoassay (InoDiag). A total of 405 patients with (n = 251) and without (n = 154) miscarriages were tested by all five tests. The prevalence of C. trachomatis-specific IgG antibodies as determined by the IF, cHSP60-Medac, MOMP-Medac, MOMP-RB and InoDiag was 14.3, 23.2, 14.3, 11.9 and 26.2%, respectively. InoDiag exhibited the highest sensitivity, whereas MOMP-RB showed the best specificity. Cross-reactivity was observed with C. pneumoniae using IF, MOMP-RB and InoDiag, and Parachlamydia acanthamoebae using the cHSP60 ELISA test. No cross-reactivity was observed between C. trachomatis and the other Chlamydiales (Neochlamydia hartmannellae, Waddlia chondrophila and Simkania negevensis). Given its high sensitivity, the new automated epifluorescence immunoassay from InoDiag represents an interesting alternative. The MOMP-based ELISA of R-Biopharm should be preferred for large serological studies, given the high throughput of ELISA and its excellent specificity.