Opportunities for new studies of nuclear DNA replication enzymology in budding yeast.
Opportunities for new studies of nuclear DNA replication enzymology in budding yeast.
复制标题
芽殖酵母核 DNA 复制酶学新研究的机会。
DOI：
10.1007/s00294-019-01023-4
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发表时间：
2020
期刊：
影响因子：
2.5
通讯作者：
Kunkel,ThomasA
中科院分区：
文献类型：
--
作者：
Garbacz,MartaA;Lujan,ScottA;Kunkel,ThomasA
三种主要的真核 DNA 聚合酶，聚合酶 α、δ 和 ε（Pols α、δ 和 ε），准确而高效地执行复制叉处 DNA 合成的基本过程。为了了解聚合酶使用的必要性和灵活性，我们最近报道，缺乏 Pol ε 核酸外切酶和聚合酶结构域 (pol2-16) 的芽殖酵母细胞能够存活，但存在严重的生长缺陷、检查点激活、dNTP 库水平增加以及突变率显着增加。在此，我们提出了新的机会来区分 Pol ε 与其他两种真核 B 家族 DNA 聚合酶 Pols δ 和 ζ 的作用。
Three major eukaryotic DNA polymerases, Polymerases α, δ, and ε (Pols α, δ, and ε), perform the fundamental process of DNA synthesis at the replication fork both accurately and efficiently. In trying to understand the necessity and flexibility of the polymerase usage, we recently reported that budding yeast cells lacking Pol ε exonuclease and polymerase domains (pol2-16) survive, but have severe growth defects, checkpoint activation, increased level of dNTP pools as well as significant increase in the mutation rates. Herein, we suggest new opportunities to distinguish the roles of Pol ε from those of two other eukaryotic B-family DNA polymerases, Pols δ and ζ.