Islet capillary blood pressure increase mediated by hyperglycemia in NIDDM GK rats
Islet capillary blood pressure increase mediated by hyperglycemia in NIDDM GK rats
复制标题
DOI：
10.2337/diabetes.46.6.947
复制
发表时间：
1997-06-01
期刊：
影响因子：
7.7
通讯作者：
Kallskog, O
中科院分区：
文献类型：
--
作者：
Carlsson, PO;Jansson, L;Kallskog, O
本研究旨在测量正常大鼠在基础条件下和急性葡萄糖刺激胰岛素释放后的胰岛毛细血管压力。此外，在GK大鼠（NIDDM动物模型）中估计胰岛毛细血管压力。采用显微穿刺技术直接测量单个胰岛毛细血管的流体静压。通过注射中性红使胰岛可视化。这种活体染色对胰岛功能、整个胰腺和胰岛血流量以及外分泌胰腺中的毛细血管血压没有影响。血糖正常的Wistar F大鼠的胰岛毛细血管血压估计为3.1 +/- 0.3 mmHg（n = 15）。给予D-葡萄糖（1 g/kg）使该值加倍，而注射等摩尔剂量的不可代谢的葡萄糖衍生物3-O-甲基葡萄糖后未见效果。与对照Wistar F大鼠相比，GK大鼠的基础胰岛毛细血管血压升高（5.7 +/- 0.4 mmHg; n = 10; P < 0.001）。根皮苷治疗GR大鼠血糖水平的降低表明，GR大鼠基础胰岛毛细血管压力的增加是葡萄糖依赖性的和可逆的。在本研究中，我们第一次发现急性和慢性高血糖都会增加胰岛毛细血管压力。长期胰岛毛细血管压力增加对胰岛功能的影响仍有待确定。
This study was performed to measure pancreatic islet capillary pressure under basal conditions and after an acute glucose stimulation of insulin release in normal rats. In addition, the islet capillary pressure was estimated in GK rats, an animal model of NIDDM. Hydrostatic pressure in single pancreatic islet capillaries was determined in vivo by direct measurement using the micropuncture technique. The pancreatic islets were visualized by injection of neutral red. This intravital staining had no effect on islet function, whole pancreatic and islet blood flow, and capillary blood pressure in the exocrine pancreas. Islet capillary blood pressure in normoglycemic Wistar F rats was estimated at 3.1 +/- 0.3 mmHg (n = 15). Administration of D-glucose (1 g/kg) doubled this value, whereas no effect was seen after injection of an equimolar dose of the non-metabolizable glucose-derivative 3-O-methyl glucose. In GK rats, basal islet capillary blood pressure was increased (5.7 +/- 0.4 mmHg; n = 10; P < 0.001) when compared with the control Wistar F rats. Reduction of blood glucose levels in GR rats with phlorizin treatment showed this increased basal islet capillary pressure in GR rats to be glucose dependent and reversible. In the present study, we have for the first time shown that both acute and chronic hyperglycemia augment islet capillary pressure. The effects of a chronically increased islet capillary pressure on long-term islet function remain to be determined.