A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution.

A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution.
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DOI:
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发表时间:
2000-04
期刊:
影响因子:
3.3
通讯作者:
K. Naruse;S. Fukamachi;H. Mitani;M. Kondo;Tomoko Matsuoka;S. Kondo;Nana Hanamura;Yutaka Morita;Kazuhito Hasegawa;R. Nishigaki;A. Shimada;H. Wada;Takehiro G. Kusakabe;N. Suzuki;M. Kinoshita;A. Kanamori;T. Terado;H. Kimura;M. Nonaka;A. Shima
K. Naruse;S. Fukamachi;H. Mitani;M. Kondo;Tomoko Matsuoka;S. Kondo;Nana Hanamura;Yutaka Morita;Kazuhito Hasegawa;R. Nishigaki;A. Shimada;H. Wada;Takehiro G. Kusakabe;N. Suzuki;M. Kinoshita;A. Kanamori;T. Terado;H. Kimura;M. Nonaka;A. Shima
中科院分区:
生物学2区
文献类型:
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作者:
K. Naruse;S. Fukamachi;H. Mitani;M. Kondo;Tomoko Matsuoka;S. Kondo;Nana Hanamura;Yutaka Morita;Kazuhito Hasegawa;R. Nishigaki;A. Shimada;H. Wada;Takehiro G. Kusakabe;N. Suzuki;M. Kinoshita;A. Kanamori;T. Terado;H. Kimura;M. Nonaka;A. Shima

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我们绘制了633个标记(488个AFLP,28个RAPD,34个IRSs,75个EST,4个STS和4个表型标记)的青鳉,硬骨鱼目。使用来自回交后代的39个细胞系的参考分型DNA组确定连锁。该小组为绘图数据的积累提供了无限的DNA。青鳉染色体图谱总长度为1354.5cM,共检测到24个连锁群,与青鳉的单倍体染色体数目相对应。每个连锁群共获得13 ~ 49个标记。青鳉和斑马鱼之间的保守共线性观察到2个独立的连锁群。然而,与斑马鱼不同,青鳉连锁图谱显示出明显的重组限制在含有雄性决定区(Y)位点的连锁群上,与常染色体相比。
We mapped 633 markers (488 AFLPs, 28 RAPDs, 34 IRSs, 75 ESTs, 4 STSs, and 4 phenotypic markers) for the Medaka Oryzias latipes, a teleost fish of the order Beloniformes. Linkage was determined using a reference typing DNA panel from 39 cell lines derived from backcross progeny. This panel provided unlimited DNA for the accumulation of mapping data. The total map length of Medaka was 1354.5 cM and 24 linkage groups were detected, corresponding to the haploid chromosome number of the organism. Thirteen to 49 markers for each linkage group were obtained. Conserved synteny between Medaka and zebrafish was observed for 2 independent linkage groups. Unlike zebrafish, however, the Medaka linkage map showed obvious restriction of recombination on the linkage group containing the male-determining region (Y) locus compared to the autosomal chromosomes.