Consistency of low bone density across bone sites in SAMP6 laboratory mice
Consistency of low bone density across bone sites in SAMP6 laboratory mice
复制标题
DOI：
10.1007/s00774-003-0471-1
复制
发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Hosokawa, M
中科院分区：
文献类型：
--
作者：
Kasai, S;Shimizu, M;Hosokawa, M
骨密度测定的发展已经清楚地表明，在给定个体的不同测量部位存在骨密度差异。本研究检查了一种实验室小鼠（加速衰老小鼠; SAM）不同部位骨密度测量的一致性。通过双能X射线吸收测定法（DXA）对SAMP 6（P6）小鼠进行骨密度的系统评价，SAMP 6（P6）小鼠是一种具有低峰值骨密度的品系，通过股骨的显微光密度测定法测量，而SAMP 2（P2）和SAMR 1（R1）品系具有高峰骨密度。我们修改了Jilka的方法，以更全面地测量整个身体和其他感兴趣的区域（ROI;头部，右前腿，左前腿，右后腿，左后腿，脊柱和尾部）。总（全身）骨密度的年龄相关性变化在所研究的品系中显示出共同的模式，并且在4月龄时观察到峰值。P6骨密度峰值最低。4个月龄时P6和P2之间骨密度的详细比较显示，在所有7个ROI中，P6的区域BMD值显著较低。BMD的应变差异不能归因于尺寸差异。总之，P6小鼠不仅在股骨，而且在子区域和整个身体都表现出低骨密度。该菌株可用于研究老年性骨质疏松症的遗传基础。
The development of bone densitometry has made it clear that there are discrepancies in bone density at various measurement sites in a given individual. This study examined the consistency of bone density measurements across various sites in a strain of laboratory mouse (senescence-accelerated mouse; SAM). A systemic evaluation of the bone density was performed by dual-energy X-ray absorptiometry (DXA) on SAMP6 (P6) mice, a strain with low peak bone density, as measured by microphotodensitometry of the femoral bones, whereas the SAMP2 (P2) and SAMR1 (R1) strains have high peak bone density. We modified Jilka's method to more comprehensively measure the whole body and additional regions of interest (ROIs; head, right foreleg, left foreleg, right hindleg, left hindleg, spine, and tail). The age-related changes in the total (whole-body) BMD showed a common pattern among the strains studied, and the peak value was seen at 4 months old. P6 showed the lowest peak BMD. A detailed comparison of the bone density between P6 and P2 at the age of 4 months revealed significantly lower regional BMD values for P6 in all seven ROIs. The strain difference in BMD could not be attributed to a difference in size. In conclusion, P6 mice showed low bone density not only in their femurs but also in the subregions and over their entire body. This strain can be potentially useful in the investigation of the genetic basis of senile osteoporosis.