Increase in expression of brain serotonin transporter and monoamine oxidase A genes induced by repeated experience of social defeats in male mice
Increase in expression of brain serotonin transporter and monoamine oxidase A genes induced by repeated experience of social defeats in male mice
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DOI：
10.1023/a:1015238124000
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发表时间：
2002-04-01
影响因子：
2.8
通讯作者：
Kudryavtseva, NN
中科院分区：
文献类型：
--
作者：
Filipenko, ML;Beilina, AG;Kudryavtseva, NN
5-羟色胺转运体和单胺氧化酶A参与5-羟色胺的失活。前者负责从突触再摄取5-羟色胺，而后者催化突触前末梢的5-羟色胺脱氨。本研究观察了CBA/Lac雄性小鼠中缝核群5-羟色胺转运体和单胺氧化酶A（MAO A）基因的表达。用多重RT-PCR方法检测这些基因的cDNA表达量。两个独立的实验表明，被击败的小鼠的特点是显着更高水平的5-羟色胺转运蛋白和单胺氧化酶A的mRNA比对照组和侵略性的动物。MAO A和5-羟色胺转运体基因表达的增加反映了社会应激诱导的β-羟色胺能系统功能激活后5-羟色胺降解的加速。MAO A和5-羟色胺转运蛋白mRNA水平之间的显著正相关表明这些基因的转录活性调节的共同途径。
Serotonin transporter and monoamine oxidase (MAO) A are involved in the inactivation of serotonin. The former is responsible for serotonin re-uptake from the synapse, whereas the latter catalyzes serotonin deamination in presynaptic terminals. Expression of serotonin transporter and MAO A genes was investigated in raphe nuclei of midbrain of CBA/Lac male mice with repeated experience of social victories or defeats in 10 daily aggressive confrontations. The amount of cDNA of these genes was evaluated using multiplex RT-PCR. Two independent experiments revealed that the defeated mice were characterized by significantly higher levels of serotonin transporter and MAO A mRNAs than the control and aggressive animals. Increased expression of MAO A and serotonin transporter genes is suggested to reflect the accelerated serotonin degradation in response to activation of the serotonergic system functioning induced by social stress. Significant positive correlation between MAO A and serotonin transporter m RNA levels suggests common pathways of regulation of transcriptional activity of these genes.