Correlative instrumental neutron activation analysis, light microscopy, transmission electron microscopy, and X-ray microanalysis for qualitative and quantitative detection of colloidal gold spheres in biological specimens
Correlative instrumental neutron activation analysis, light microscopy, transmission electron microscopy, and X-ray microanalysis for qualitative and quantitative detection of colloidal gold spheres in biological specimens
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DOI：
10.1017/s143192769898045x
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发表时间：
1998-09-01
影响因子：
2.8
通讯作者：
Albrecht, RM
中科院分区：
文献类型：
--
作者：
Hillyer, JF;Albrecht, RM
胶体金与配体或抗体结合，通常用作通过光镜(LM)和电子显微镜(EM)检测细胞结构的标记。到目前为止，使用了几种方法来计算胶体金标签的数量，但收效甚微。仪器中子活化分析(INAA)是一种分析材料元素组成的物理方法，可以用来提供大量样品中金积累的定量指标。鉴于生物样品中不存在大量的金，而且可以制备胶体金和配体结合物以产生均匀的珠子大小，因此可以通过INAA计算大量生物样品中的标记量。在这里，我们描述了在模型中使用INAA、LM、透射EM和X射线显微分析(EDX)来确定在给药后在整个动物系统中器官、组织、细胞和超微结构水平的胶体金的分布(定位)和数量。并与电感耦合等离子体质谱(ICPMS)测定生物样品中金的灵敏度进行了比较。INAA、LM、TEM和EDX的相关使用可能是有用的，例如，在体内给药后对各种标记分子物种的定量和定性跟踪。
Colloidal gold, conjugated to ligands or antibodies, is routinely used as a label for the detection of cell structures by light (LM) and electron microscopy (EM). To date, several methods to count the number of colloidal gold labels have been employed with limited success. Instrumental neutron activation analysis (INAA), a physical method for the analysis of the elemental composition of materials, can be used to provide a quantitative index of gold accumulation in bulk specimens. Given that gold is not naturally found in biological specimens in any substantial amount and that colloidal gold and ligand conjugates can be prepared to yield uniform bead sizes, the amount of label can be calculated in bulk biological samples by INAA. Here we describe the use of INAA, LM, transmission EM, and X-ray microanalysis (EDX) in a model to determine both distribution (localization) and amount of colloidal gold at the organ, tissue, cellular, and ultrastructural levels in whole animal systems following administration. In addition, the sensitivity for gold in biological specimens by INAA is compared with that of inductively coupled plasma-mass spectrometry (ICP-MS). The correlative use of INAA, LM, TEM, and EDX can be useful, for example, in the quantitative and qualitative tracking of various labeled molecular species following administration in vivo.