Induction of lytic cycle replication of Kaposi's sarcoma-associated herpesvirus by herpes simplex virus type 1: involvement of IL-10 and IL-4
Induction of lytic cycle replication of Kaposi's sarcoma-associated herpesvirus by herpes simplex virus type 1: involvement of IL-10 and IL-4
复制标题
1 型单纯疱疹病毒诱导卡波西肉瘤相关疱疹病毒的裂解周期复制：IL-10 和 IL-4 的参与
DOI：
10.1111/j.1462-5822.2007.01079.x
复制
发表时间：
2008-03-01
影响因子：
3.4
通讯作者：
Lu, Chun
中科院分区：
文献类型：
--
作者：
Qin, Di;Zeng, Yi;Lu, Chun
此前，我们发现人类疱疹病毒6型和人类免疫缺陷病毒1型Tat都是激活卡波西肉瘤相关疱疹病毒（KSHV）裂解周期复制的重要辅助因子。在这里，我们进一步研究了 1 型单纯疱疹病毒 (HSV-1) 影响 KSHV 复制的潜力。我们证明，HSV-1 是卡波西肉瘤发病机制中的一个潜在重要因素，这通过 BCBL-1 细胞中裂解期 mRNA 转录物、病毒蛋白和感染性病毒颗粒的产生来确定。这些结果通过使用靶向 KSHV ORF50 的小干扰 RNA 的 RNA 干扰实验和测试 ORF50 启动子驱动的荧光素酶活性的荧光素酶报告基因测定进一步证实。最后，我们发现人白介素 10 (IL-10) 和 IL-4 的产生部分促进了 HSV-1 诱导的 KSHV 复制。我们的数据首次提供了 HSV-1 可以激活 KSHV 裂解性复制的直接证据，并表明 HSV-1 在 KSHV 发病机制中的作用。
Previously, we identified that both human herpesvirus 6 and human immunodeficiency virus type 1 Tat were important cofactors that activated lytic cycle replication of Kaposi's sarcoma-associated herpesvirus (KSHV). Here, we further investigated the potential of herpes simplex virus type 1 (HSV-1) to influence KSHV replication. We demonstrated that HSV-1 was a potentially important factor in the pathogenesis of Kaposi's sarcoma, as determined by production of lytic phase mRNA transcripts, viral proteins and infectious viral particles in BCBL-1 cells. These results were further confirmed by an RNA interference experiment using small interfering RNA targeting KSHV ORF50 and a luciferase reporter assay testing ORF50 promoter-driven luciferase activity. Finally, we discovered that production of human interleukin-10 (IL-10) and IL-4 partially contributed to HSV-1-induced KSHV replication. Our data present the first direct evidence that HSV-1 can activate KSHV lytic replication and suggest a role of HSV-1 in KSHV pathogenesis.