Detection of strain specific double-stranded RNAs in citrus species infected with citrus tristeza virus: a review 313KB)
Detection of strain specific double-stranded RNAs in citrus species infected with citrus tristeza virus: a review 313KB)
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感染柑橘 tristeza 病毒的柑橘品种中品系特异性双链 RNA 的检测：综述 313KB）
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Lee
中科院分区：
文献类型：
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作者：
J. Dodds;Jarupat;Roistacher;Lee
当通过聚丙烯酰胺凝胶电泳分析从 2.0 g 受感染树皮组织中纯化的 dsRNA 时，检测到柑橘 tristeza 病毒 (CTV) 的主要和次要 dsRNA，并与置信度进行比较。在甜橙、酸橙、墨西哥酸橙、柠檬、葡萄柚、香橼和柑橘中检测到病毒特异性 dsRNA。最可靠的 dsRNA 结果是从甜橙、C. excelsa 和香橼中获得的。 dsRNA 数量的排名通常与测试宿主中 CTV 的抗原滴度相匹配。四种菌株的 dsRNA 谱总体上各具特色，但葡萄柚和柠檬往往会影响 dsRNA 的质量和数量。在一年中白天温度最高的月份中，DsRNA 的检测很困难。无法检测到 0.5 x l<r MW dsRNA 与分离株的生物学名称（既不是葡萄柚幼苗黄化型，也不是葡萄柚茎点蚀分离株）之间存在很强的相关性。
Major and minor dsRNAs of citrus tristeza virus (CTV) were detected and compared with confidence when the dsRNA purified from 2,0 g of infected bark tissue was analysed by polyacrylamide gel electrophoresis. Virus specific dsRNAs were detected in sweet orange, sour orange, Mexican lime, lemon, grapefruit, citron, and Citrus excelsa. The most reliable dsRNA results were obtained from sweet orange, C. excelsa and citron. Rankings for dsRNA quantity were generally matched by the antigen titre of CTV in the hosts tested. The dsRNA profiles were generally characteristic for each of four strains, but grapefruit and lemon had a tendency to affect the quality, as well as the quantity of dsRNA. DsRNA was detected with diffIculty in the months of the year when daytime temperatures were highest. There was a strong correlation between inability to detect a 0,5 x l<r MW dsRNA and the biological designation of an isolate as neIther a seedling yellows type, nor a stem pitting isolate of grapefruit.