Splice Variant Identification and Expression Analysis of the Fat Mass and Obesity-Associated (FTO) Gene in Intact and Castrated Male Pigs
Splice Variant Identification and Expression Analysis of the Fat Mass and Obesity-Associated (FTO) Gene in Intact and Castrated Male Pigs
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DOI：
10.1089/dna.2009.1004
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发表时间：
2010-12-01
影响因子：
3.1
通讯作者：
Fang, Meiying
中科院分区：
文献类型：
--
作者：
Huang, Jinming;Liu, Gang;Fang, Meiying
脂肪量和肥胖相关基因（FTO）先前已与人体质量指数和猪肌内脂肪含量相关。在这项研究中，潜在的选择性剪接FTO基因的模式进行了调查，并确定了三个新的剪接变异体在大白色和本土中国藏猪。为探讨FTO基因在去势引起的脂肪沉积中的分子效应，将40对全同胞公猪分为正常组和去势组，性状数据显示，正常组的腹脂重、腹脂重百分比和背膘厚显著低于去势组（p < 0.05）。去势组下丘脑FTO mRNA表达显著高于正常组（p < 0.05），而腹脂和背脂组织FTO mRNA表达无显著差异。此外，与脂肪组织中的表达相比，FTO基因的相对表达在下丘脑中显示出更高的水平（p < 0.05）。我们的研究结果表明，有不同的模式的选择性剪接和FTO的表达猪品种。本研究为进一步了解猪FTO基因的功能提供了线索。
The fat mass and obesity-associated gene (FTO) has previously been associated with human body mass index and porcine intramuscular fat content. In this study, the potential alternative splicing pattern of the FTO gene was investigated, and three novel splice variants were identified in Large White and indigenous Chinese Tibetan pigs. To explore the molecular effect of the FTO gene on the fat deposition caused by castration, 40 paired full-sibling male pigs were divided into intact and castrated groups, and the trait data showed that abdominal fat weight, percentage of abdominal fat weight, and backfat thickness in the intact group were significantly lower than in the castrated group (p < 0.05). FTO mRNA expression in the hypothalamus was significantly increased in the castrated group compared with the intact group (p < 0.05), but was not different in the abdominal fat or backfat tissues. Moreover, the relative expression of the FTO gene was shown to have a higher level in the hypothalamus when compared with expression in the fat tissues (p < 0.05). Our results suggest that there are different patterns of alternative splicing and FTO expression among pig breeds. This study will provide clues for obtaining a better understanding of the porcine FTO gene function.