Unusually high frequencies of HIV-specific cytotoxic T lymphocytes in humans.
Unusually high frequencies of HIV-specific cytotoxic T lymphocytes in humans.
复制标题
人类中 HIV 特异性细胞毒性 T 淋巴细胞的出现频率异常高。
DOI：
10.4049/jimmunol.142.2.452
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发表时间：
1989
影响因子：
4.4
通讯作者：
F. Plata
中科院分区：
文献类型：
--
作者：
A. Hoffenbach;P. Langlade‐Demoyen;G. Dadaglio;E. Vilmer;F. Michel;C. Mayaud;B. Autran;F. Plata
HIV特异性CTL属于T淋巴细胞的CD8亚群，其活性受到I类HLA移植Ag的限制。在本报告中，hiv特异性CTL及其前体细胞通过限制稀释分析进行了定量。从无症状血清阳性携带者和艾滋病患者的肺、淋巴结和血液中回收CTL。人们发现艾滋病毒具有很强的免疫原性。在感染个体中检测到hiv特异性CTL和CTL前体细胞的高频率。这些CTL杀死了自体hiv感染的巨噬细胞和T4淋巴细胞。他们还杀死了双重转染的P815-A2-env-LAV小鼠肿瘤细胞，这些细胞表达人类HLA-A2基因和HIV-1 env基因。在对两名hiv感染患者的纵向研究中，CTL和CTL前体细胞频率随着患者临床和免疫状况的恶化而下降。最令人惊讶的是，来自血清阴性供体的PBL也对体外的HIV刺激有反应，并产生大量受hla限制的HIV特异性CTL。
CTL specific for the HIV belong to the CD8 subset of T lymphocytes, and their activity is restricted by class I HLA transplantation Ag. In this report, HIV-specific CTL and their precursor cells were quantified by limiting dilution analysis. CTL were recovered from the lungs, lymph nodes, and blood of asymptomatic seropositive carriers and of patients with AIDS. HIV was found to be very immunogenic. High frequencies of both HIV-specific CTL and CTL precursor cells were detected in infected individuals. These CTL killed autologous HIV-infected macrophages and T4 lymphoblasts. They also killed doubly transfected P815-A2-env-LAV mouse tumor cells, which express the human HLA-A2 gene and the HIV-1 env gene. In the longitudinal studies of two HIV-infected patients, CTL and CTL precursor cell frequencies decreased as the clinical and immunologic status of the patients deteriorated. Most surprisingly, PBL from seronegative donors also responded to HIV stimulation in vitro and generated large numbers of HLA-restricted, HIV-specific CTL.