A GRADIENT OF BICOID PROTEIN IN DROSOPHILA EMBRYOS
A GRADIENT OF BICOID PROTEIN IN DROSOPHILA EMBRYOS
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DOI：
10.1016/0092-8674(88)90182-1
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发表时间：
1988-07-01
期刊：
影响因子：
64.5
通讯作者：
NUSSLEINVOLHARD, C
中科院分区：
文献类型：
--
作者：
DRIEVER, W;NUSSLEINVOLHARD, C
母体双体基因（bcd）组织果蝇的前发育。其mRNA定位于卵母细胞和早期胚胎的前尖端。在0-4小时胚提取物的Western blots中，bcd融合蛋白抗体可识别55-57 kd的双线带。这种蛋白在11个bcd等位基因中的9个胚胎提取物中缺失或减少。这种蛋白质集中在卵裂期胚胎的细胞核中。在卵母细胞中检测不到，表明bcd mRNA翻译受时间控制。bcd蛋白呈指数浓度梯度分布，在胚胎前尖处达到最大值，在胚胎后三分之一处达到本底水平。梯度可能是由局部mRNA源的扩散和分散降解产生的。
The maternal gene bicoid (bcd) organizes anterior development in Drosophila. Its mRNA is localized at the anterior tip of the oocyte and early embryo. Antibodies raised against bcd fusion proteins recognize a 55-57 kd doublet band in Western blots of extracts of 0-4 hr old embryos. This protein is absent or reduced in embryonic extracts of nine of the 11 bcd alleles. The protein is concentrated in the nuclei of cleavage stage embryos. It cannot be detected in oocytes, indicating temporal control of bcd mRNA translation. The bcd protein is distributed in an exponential concentration gradient with a maximum at the anterior tip, reaching background levels in the posterior third of the embryo. The gradient is probably generated by diffusion from the local mRNA source and dispersed degradation.