Temporal trends in antibody production in captive grey, harp and hooded seals to a single administration immunocontraceptive vaccine
Temporal trends in antibody production in captive grey, harp and hooded seals to a single administration immunocontraceptive vaccine
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DOI：
10.1016/s0165-0378(97)00048-x
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发表时间：
1997-11-01
影响因子：
3.4
通讯作者：
Pohajdak, B
中科院分区：
文献类型：
--
作者：
Brown, RG;Bowen, WD;Pohajdak, B
在圈养灰海豹(Phoca Groenlandica)、竖琴海豹(Phoca Groenlandica)和冠海豹(Cystophora Cristata)身上，研究了单剂免疫避孕疫苗在自由放养的雌性灰海豹(Halichoerus Grypus)体内产生抗体的时间。该疫苗是基于脂质体输送猪卵透明带抗原的。当通过抗原捕获来测量时，帽海豹和竖琴海豹对疫苗的反应与灰海豹的反应相似。用A蛋白、兔抗海豹免疫球蛋白血清和亲和层析后的SDS-PAGE检测抗体产生，证实了灰海豹和竖海豹对疫苗的反应相似，但白头海豹的效价较低。该疫苗在圈养海豹、幼年灰海豹和竖琴海豹中产生效价，已知在野生成年灰海豹中具有免疫避孕作用。(C)1997年爱思唯尔爱尔兰科学有限公司。
The temporal production of antibody to a single-administration immunocontraceptive vaccine, known to be immunocontraceptive in free-ranging female grey seals (Halichoerus grypus), was studied in captive grey seals, harp seals (Phoca groenlandica) and hooded seals (Cystophora cristata). The vaccine is based on liposome delivery of porcine zona pellucida antigens. When measured by antigen capture, the response of hooded and harp seals to the vaccine was similar to the response of grey seals. Determination of antibody production by ELISA with protein A, ELISA with rabbit anti-seal immunoglobulin sera and SDS-PAGE after affinity chromatography confirmed the similarity in response to the vaccine by grey and harp seals, but suggested lower titers in hooded seals. The vaccine produced titers in captive, juvenile grey and harp seals known to be immunocontraceptive in wild, adult grey seals. (C) 1997 Elsevier Science Ireland Ltd.