Effects of hyperbaric oxygen treatment on antimicrobial function and apoptosis of differentiated HL-60 (neutrophil-like) cells
Effects of hyperbaric oxygen treatment on antimicrobial function and apoptosis of differentiated HL-60 (neutrophil-like) cells
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DOI：
10.1016/j.lfs.2013.06.003
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发表时间：
2013-07-30
期刊：
影响因子：
6.1
通讯作者：
Moody, A. John
中科院分区：
文献类型：
--
作者：
Almzaiel, Anwar J.;Billington, Richard;Moody, A. John
目的：中性粒细胞凋亡在慢性创面炎症消退中起重要作用。高压氧(HBO)疗法是一种在高于大气压的情况下间歇吸入100%氧气的疗法，似乎是治疗慢性伤口的有效方法。目的：利用全反式维甲酸(ATRA)分化的HL-60细胞(中性粒细胞样细胞)，验证HBO诱导的抗菌活性增强可能导致细胞凋亡增加，从而有助于中性粒细胞从慢性创伤中清除的假设。主要方法：采用ATRA分化的HL-60细胞，体外中性粒细胞模型，检测常氧、低氧(5%O-2)、高氧(95%O-2)的影响。高压常氧(2.4ATA时8.8%O-2)和高压氧(2.4ATA时97.9%O-2)对中性粒细胞抗菌功能[NBT染色、超氧化物和过氧化氢生成、吞噬活性]和细胞凋亡(caspase3/7活性和形态变化用SYBR安全染色观察)的影响。金黄色葡萄球菌的吞噬功能也增强。HBO预处理具有促凋亡作用，使caspase3/7活性升高，并引起与细胞凋亡相关的形态变化。意义：HBO诱导的抗菌活性增强的潜在有害影响可能被增强的细胞凋亡所抵消。高氧和压力似乎单独导致了HBO诱导的抗菌活性和细胞凋亡的增加，尽管没有一致的模式。这些数据有助于解释HBO在治疗慢性伤口方面的有效性。(C)2013 Elsevier Inc.保留所有权利。
Aims: Neutrophil apoptosis is important in the resolution of inflammation in chronic wounds. Hyperbaric oxygen (HBO) therapy, an intermittent inhalation of 100% oxygen at greater than atmospheric pressure, appears to be an effective treatment for chronic wounds. The aim was to use HL-60 cells differentiated using all-trans retinoic acid (ATRA) (neutrophil-like cells) to test the hypothesis that an HBO-induced increase in antimicrobial activity might lead to an increase in apoptosis, thereby contributing to neutrophil clearance from chronic wounds.Main methods: ATRA differentiated HL-60 cells, an in vitro neutrophil model, were used to test the effects of normoxia, hypoxia (5% O-2), hyperoxia (95% O-2). hyperbaric normoxia (pressure) (8.8% O-2 at 2.4 ATA) and HBO (97.9% O-2 at 2.4 ATA) on antimicrobial function [NBT staining, superoxide and H2O2 production, and phagocytosis activity] and apoptosis (caspase 3/7 activity and morphological changes observed using SYBR Safe staining).Key findings: A single 90 min HBO exposure caused an increase in the respiratory burst activity of neutrophil-like cells post exposure. Phagocytosis of Staphylococcus aureus was also increased. HBO pre-treatment had a proapoptotic effect, increasing caspase 3/7 activity and causing morphological changes associated with apoptosis.Significance: The potential detrimental effect of enhanced antimicrobial activity induced by HBO may be offset by enhanced apoptosis. Both hyperoxia and pressure alone seemed to contribute to the HBO-induced increases in antimicrobial activity and apoptosis, although there was no consistent pattern. These data contribute to explaining the effectiveness of HBO in the treatment of chronic wounds. (C) 2013 Elsevier Inc. All rights reserved.