ALLERGENS IN HYMENOPTERA VENOM .25. THE AMINO-ACID-SEQUENCES OF ANTIGEN 5 MOLECULES AND THE STRUCTURAL BASIS OF ANTIGENIC CROSS-REACTIVITY
ALLERGENS IN HYMENOPTERA VENOM .25. THE AMINO-ACID-SEQUENCES OF ANTIGEN 5 MOLECULES AND THE STRUCTURAL BASIS OF ANTIGENIC CROSS-REACTIVITY
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DOI：
10.1016/0091-6749(93)90014-7
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发表时间：
1993-11-01
影响因子：
14.2
通讯作者：
HOFFMAN, DR
中科院分区：
文献类型：
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作者：
HOFFMAN, DR
用固相蛋白质测序法测定了8种不同水泡型蛇毒抗原5分子的完整氨基酸序列。这些物种包括五种黄色夹克，即VesPula Squamosa、V.Favopilosa、V.germanica、V.Pensylvanica和V.vidua，代表所有这三个物种组；来自欧洲大黄蜂的两个变种，Vespa Crabro；以及来自第二个亚属的纸蜂物种Polistes fuscatus。新的序列与之前发表的来自黄夹克、黄蜂和黄蜂的7个序列以及来自进口火蚁毒液的火蚁变应原3的序列进行了比较。这些比较提供了结构证据，支持观察到的黄夹克共同组的抗原和所研究的北美两个常见纸蜂亚属的抗原之间高度交叉反应。鳞状弧菌和绿色弧菌的抗原5与普通弧菌的抗原5有显著差异。可产生免疫交叉反应的共同特征是在两个属的黄蜂抗原5分子之间以及在黄夹克、黄蜂和纸黄蜂之间。进口的火蚁变应原与水泡性变应原只有最小的保守区，这就解释了为什么没有观察到IgE交叉反应。这些结果为临床上观察到的交叉反应模式提供了结构基础，并表明黄蜂和纸蜂的商业提取物可以用更少的精心挑选的物种来制备。
The complete amino acid sequences have been determined by solid-phase protein sequencing for eight different vespid venom antigen 5 molecules. These include five species of yellow jackets, Vespula squamosa, V. flavopilosa, V. germanica, V. pensylvanica and V. vidua, representing all three species groups; two variants from the European hornet, Vespa crabro; and a species of paper wasp, Polistes fuscatus, from a second subgenus. The new sequences were compared with the seven previously published sequences from yellow jackets, hornets, and wasps, and to that of Solenopsis invicta 3 allergen from imported fire ant venom. These comparisons provided structural evidence to support the observed high degree of cross-reactivity among the antigens of the common group of yellow jackets and among those of the two common North American subgenera of paper wasps studied. The antigen 5 of V. squamosa and of V. vidua were significantly different from those of the vulgaris group. Common features that could generate immunologic cross-reactivity were seen among the antigen 5 molecules of hornets of both genera and among those of yellow jackets, hornets, and paper wasps. The imported fire ant allergen has only minimal conserved areas in common with the vespid allergens, which explains the lack of observed IgE cross-reactivity. These results provide the structural basis for the cross-reactivity patterns observed in clinical practice and suggest that the commercial extracts of yellow jacket and paper wasp could be prepared with fewer carefully selected species.