Effect of uterine distension and oestrogen treatment on gap junction formation in the myometrium of the rat.
Effect of uterine distension and oestrogen treatment on gap junction formation in the myometrium of the rat.
复制标题
子宫扩张和雌激素治疗对大鼠子宫肌层间隙连接形成的影响。
DOI：
10.1530/jrf.0.0650497
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
D. G. Porter
中科院分区：
文献类型：
--
作者：
D. Wathes;D. G. Porter
子宫肌层缝隙连接在妊娠21天、22天、7天和产后1天分别为1/5、4/5、7/7和1/3。其频率(每毫米膜数)从0.30增加到2.20和6.48，然后下降到0.31。对10只去卵巢产后大鼠在一侧子宫内安装球囊，记录压力变化。对照组大鼠维持持续的压力循环。服用7.5微克雌二醇-17β的大鼠15 h后宫内压力周期的频率从约50次/小时降至4.3次/小时，但子宫压力周期的最大上升速率从7.2+/-0.95升至11.3+/-1.85毫米汞/秒-1(n=5，P<0.05)。雌激素组大鼠每毫米膜缝隙连接的总数在装有球囊的角细胞中为7.37个，在空角细胞中为1.03个，而对照组分别为0.97个和无缝隙连接细胞。雌激素治疗的子宫肌层中压力升高的速度表明细胞间的耦合得到改善，这与缝隙连接的频率较高有关。雌激素治疗和(从气球上)扩张似乎都会导致一些缝隙连接的形成，但只有在这两种治疗方法相结合的动物身上才能获得相当于分娩时的缝隙连接数量。
Gap junctions were present in the myometrium of 1 out of 5 rats on Day 21 of pregnancy, 4 out of 5 on the morning of Day 22, 7 out of 7 during parturition and 1 out of 3 on Day 1 post partum. Their frequency (number per mm membrane) at these time periods increased from 0.30 to 2.20 and 6.48 and then decreased to 0.31. Ten ovariectomized post-partum rats were fitted with an intrauterine balloon in one horn for recording pressure changes. Control rats maintained continuous pressure cycles. In rats given 7.5 micrograms oestradiol-17 beta the frequency of intrauterine pressure cycles decreased from about 50 per hour to 4.3 per hour 15 h later, but the maximum rate of rise of uterine pressure cycles increased significantly from 7.2 +/- 0.95 to 11.3 +/- 1.85 mmHg sec-1 (n = 5, P less than 0.05). The overall number of gap junctions per mm membrane in the oestrogen-treated rats was 7.37 in the horns equipped with balloons and 1.03 in the empty horns, compared with 0.97 and none respectively in the control rats. The increased rate of rise of pressure in the oestrogen-treated myometrium indicated improved coupling between cells and this was associated with a high frequency of gap junctions. Both oestrogen treatment and distension (from the balloon) appeared to cause some gap junctions to form, but numbers equivalent to those at parturition were only obtained in animals in which these two treatments were combined.